About ZR Plant RNA MiniPrep™
The ZR Plant RNA MiniPrep™ provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.). For purification of total RNA including small RNAs (~50 µg), the ZR Plant RNA MiniPrep™ features a specially formulated RNA Lysis Buffer. The Zymo-Spin™ IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IIC Column efficiently adsorbs total RNA.
The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin™ IV-HRC Spin Filters. The RNA is suitable for use in various subsequent procedures including RT-PCR.
The entire RNA isolation procedure typically takes about 10 minutes.
|Format||Bead beating, spin column|
|Equipment||Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional).|
|Sample Sources||Leaves, stems, buds, flowers, fruit, seeds, etc.|
|RNA Purity||High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.|
|RNA Recovery||RNA can be eluted into small volumes, ≥25 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of the provided column is ~50 µg.|
|Compatibility||Compatible with samples stored in RNAlater ™.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.|
Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the ZR Plant RNA MiniPrep™. Leaves were minced then processed using a FastPrep®-24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium™ Markers (Ambion) were used as size standards
Nicotiana sp. leaf samples were spiked with humic acid (Sigma) at a final Ab230nm = 0.2. Total RNA was isolated with the ZR Plant RNA MiniPrep™ with and without the use of the Zymo-Spin™ IV-HRC Spin Filter. RT-PCR performed with a LightCycler® 480 (Roche) showed an increase in fluorescence signal and detected an early amplification initiation for the Zymo-Spin™ IV-HRC treated samples compared to the non-treated samples (cp = [30 vs. 31], respectively).
|Click Here to download the protocol|