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ZR Plant RNA MiniPrep™

Quick, 10 minute isolation of inhibitor-free total RNA (~50 µg) from a wide variety of plant samples using ultra-high density BashingBeads™ and Zymo-Spin™ column technologies.
High-quality RNA eluted in ≥25 µl is ready for reverse transcription, microarray, sequencing, etc.

Product Size Catalog # Price Qty
ZR Plant RNA MiniPrep™ 50 Preps. R2024

About ZR Plant RNA MiniPrep™

The ZR Plant RNA MiniPrep provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.).  For purification of total RNA including small RNAs (~50 µg), the ZR Plant RNA MiniPrep features a specially formulated RNA Lysis Buffer.  The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently adsorbs total RNA. 

The RNA is washed and then eluted with DNase/RNase-Free Water.  For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin IV-HRC Spin Filters.  The RNA is suitable for use in various subsequent procedures including RT-PCR. 

The entire RNA isolation procedure typically takes about 10 minutes.

Format Bead beating, spin column
Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional).
Sample Sources Leaves, stems, buds, flowers, fruit, seeds, etc.
RNA Purity High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.
RNA Recovery RNA can be eluted into small volumes, ≥25 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of the provided column is ~50 µg.
Compatibility Compatible with samples stored in RNAlater ™.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.

Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the ZR Plant RNA MiniPrep™. Leaves were minced then processed using a FastPrep®-24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium™ Markers (Ambion) were used as size standards

Nicotiana sp. leaf samples were spiked with humic acid (Sigma) at a final Ab230nm = 0.2. Total RNA was isolated with the ZR Plant RNA MiniPrep™ with and without the use of the Zymo-Spin™ IV-HRC Spin Filter. RT-PCR performed with a LightCycler® 480 (Roche) showed an increase in fluorescence signal and detected an early amplification initiation for the Zymo-Spin™ IV-HRC treated samples compared to the non-treated samples (cp = [30 vs. 31], respectively).

Click Here to download the protocol
For specific notes and additional information, please see the product protocol PDF.
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