Direct-zol™ RNA Kits

Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.

Product Size Catalog # Price Qty
NEW Direct-zol™ RNA MiniPrep Plus 50 Preps R2070
$180.00
NEW Direct-zol™ RNA MiniPrep Plus 200 Preps R2072
$555.00
NEW Direct-zol™ RNA MiniPrep Plus w/ TRI Reagent® 50 Preps R2071
$250.00
NEW Direct-zol™ RNA MiniPrep Plus w/ TRI Reagent® 200 Preps R2073
$770.00
Direct-zol™ RNA MiniPrep 50 Preps R2050
$180.00
Direct-zol™ RNA MiniPrep 200 Preps R2052
$555.00
Direct-zol™ RNA MiniPrep w/ TRI-Reagent® 50 Preps R2051
$250.00
Direct-zol™ RNA MiniPrep w/ TRI-Reagent® 200 Preps R2053
$770.00
NEW Direct-zol™ RNA MicroPrep 50 Preps R2060
$175.00
NEW Direct-zol™ RNA MicroPrep 200 Preps R2062
$545.00
NEW Direct-zol™ RNA MicroPrep w/ TRI Reagent® 50 Preps R2061
$245.00
NEW Direct-zol™ RNA MicroPrep w/ TRI Reagent® 200 Preps R2063
$762.00

About Direct-zol™ RNA Kits

  MiniPrep Plus MiniPrep MicroPrep
RNA Recovery 100 µg 50 µg 10 µg
Minimum Elution 50 µl 25 µl 6 µl
(Animal) Cells ≤ 107 ≤ 5 x 106 ≤ 106
Tissue ≤ 50 mg ≤ 25 mg ≤ 5 mg

The Direct-zol RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar.  Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).

Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis.  The Direct-zol method assures unbiased recovery of small RNAs including miRNA (see below).

The procedure is easy.  Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA.  No phase separation, precipitation, or post-purification steps are necessary.  The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).

The entire procedure typically takes only 7 minutes.

*U.S. Patent No. 9,051,563 and other pending patents.


Equipment Microcentrifuge
Sample Sources Any sample stored and preserved in TRI Reagent®, TRIzol® or similar. (animal cells, tissue, bacteria, yeast, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
RNA Purity A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA can be accomplished using an in-column DNase I digestion
Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
RNA Storage RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors is highly recommended for prolonged storage.
RNA Size Limits RNAs ≥17 nucleotides.
Sample Inactivation TRI Reagent® (provided with R2051, R2053, R2061, R2063, R2071, R2073 only) inhibits RNase activity and inactivates viruses and other infectious agents.

High quality broad range RNA is purified with the Direct-zol™ RNA MiniPrep. (A) DNA-free RNA purified from human epithelial cells using the Direct-zol™ RNA MiniPrep compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). (B) Small RNAs are effectively recovered with the Direct-zol™ procedure while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown) The data show RNA purified from TRIzol® samples using the Direct-zol™ RNA MiniPrep compared to an unbiased method (mirVana™, Ambion). Micro-RNA analysis was performed using miRNA-Seq (MiSeq®, Illumina) and a direct hybridization assay (nCounter®, Nanostring).
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Featured Citations

Researchers used the Direct-zol™ RNA MiniPrep for heterologous E. coli expression of the FnCpf1 locus by means of RNA-seq and discovered a new endonuclease, Cpf1, which further improves upon the widely used CRISPR-Cas9 genome editing system. Cpf1 targets distinct PAM sequences, requires no tracrRNA, and cleaves DNA with staggered overhangs.
RNA from primary fibroblasts originating from Aicardi-Goutières syndrome (AGS) patients was purified using the Direct-zol™ RNA MiniPrep and subsequently used for reverse transcription to cDNA and RT-qPCR. Results showed that the genomes of AGS patients with TREX1, RNASEH2, and SAMHD1 mutations experience significant RNA:DNA hybrids accumulation.
Scientists used the Direct-zol RNA MiniPrep kit to investigate the role of miRNAs in an alcohol-induced inflammatory response in the brains of mice. They found that one miRNA in particular, miR-155, is upregulated following chronic ethanol feeding and plays an important role in neuroinflammation. Furthermore, the induction of miR-155 was dependent on Toll-like receptor 4 (TLR4), and mice with miR-155 or TLR4 knockouts were protected from alcohol-induced inflammation, highlighting possible pathways for alcohol abuse therapy.
Researchers used the Direct-zol RNA MiniPrep kit to identify that Kv1.1, an essential voltage-gated potassium channel that controls action potentials in neurons, can be repressed by a specific miRNA, miR-129, and that this regulatory mechanism is dependent on the activity of the kinase mTORC1. When the mTORC1 complex is catalytically active, the translation of Kv1.1 is repressed by miR-129. However, when mTORC1 activity is inhibited, translation of Kv1.1 is enhanced due to the presence of HuD, an RNA-binding protein, at the miR-129 binding sites in the Kv1.1 mRNA, preventing miRNA binding and inhibition. These results have important implications for the regulation of learning and memory.
In synthetic biology, experiments are often designed to express genes in a controllable manner so that researchers can manipulate the system to meet their needs. Researchers in Germany recently developed a mechanism to control translation in E. coli using a modified hammerhead ribozyme and small trans-acting RNAs, and they used the Direct-zol RNA MiniPrep kit in their studies. This RNA-mediated system can potentially be expanded to regulate the expression of many genes in a complex synthetic biology network.
Researchers used the Direct-zol RNA MiniPrep kit from Zymo Research to purify virally-expressed microRNAs and demonstrated that microRNAs play critical roles in regulating polyomavirus replication.
Total RNA was extracted from human, rat, and mouse cortical neurons using the Direct-zol™ RNA MiniPrep Kit. The high-quality RNA was reverse transcribed into cDNA and used to investigate the effect of BPA exposure on neurodevelopment by real-time PCR analysis.
High-quality RNA isolated with the Direct-zol™ RNA MiniPrep from fecal and culture samples were shown to be instrumental in the development of a rotavirus early detection system using RT-PCR. The efficient RNA isolation helps in identification of severe gastroenteritis in infants and young children.
The Direct-zol™ RNA MiniPrep isolated RNA from Vibrio cholerae has been used for next-gen sequencing, ChIP-Seq, RNA-Seq, qPCR and Northern-blot analysis. The high-quality RNA helped to characterize gene expression profiles of virulence factors in RpoN regulon of the cholera pathogen.
The Direct-zol RNA Miniprep kit was used to isolate pure total RNA from Rat cells, and the high quality RNA was used to create a cDNA library for RT-qPCR analysis of several genes related to hematopoietic Stem Cell (HSC) Development. The consistent RNA extraction allowed the scientists to determine that HSC cells do not significantly contribute to kidney repair following acute kidney injuries.

"Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. I tried the Zymo kit and I was definitely hesitant and did not have high hopes for it. I was wrong.....the Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench. Thank you Zymo!"
A. Newhart (The Wistar Institute)
“I am very impressed with the RNA yield (quality and quantity) I obtained using this kit. When using other methods and kits for RNA isolation- despite us taking several steps and measures to reduce RNA degradation- I found it very challenging to obtain good quality RNA. With Direct-zol RNA mini prep I was able to obtain superior quality RNA repeatedly and with ease. This kit is suitable for both small scale and high throughput RNA isolations.”
Subashini N. (Wayne State University)
“The protocol very straight forward, time needed to get the RNA is just outstanding and saved us a lot of time to do more things. The yield and the purity of the RNA is something out of this world, about at least 50% more RNA and purity not less than 1.95, we never got [that] before with other vendors such Invitrogen, Qiagen, etc.”
Ehab S. (University of Iowa)
“I liked that it wasn't necessary to separate phases with Chloroform - I think I got less gDNA contamination as a result. The option for performing an on-column DNAse1 digestion is perhaps the most significant benefit compared to doing ChCl3 separations and subsequent precipitations”
Nick A. (AgResearch, New Zealand)
“It is very easy to use and centrifugations are short. The resin is compact and absorbent which makes it easy to cover the whole resin surface with small amount of water in the elution step. Recovery of small RNAs is very good based on CT values obtained from qPCR using RNA template derived from this kit.”
Janne T. (Universidad de Zaragoza)
“The speed of the process is by far the best aspect of this kit. Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? What I also really liked is the ease of the whole process: no messing around with several buffers or different columns that get switched up. Just one column and two buffers, I love it. Oh, and the RNA yield was great too.”
Arjan V. (Indiana University)
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
Adina B. (University of Guelph)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
Mohan K. (University of Illinois, Chicago)
“Easy to use, equal results as a much more expensive kit from another company.”
Patricia O. (University of Porto)
"I've been performing RNA isolation on dozens of samples using the manual method, and this kit is amazing. It really is true to its prep time: 10 minutes. It's so much easier, and given all the other experimental methods I need to perform, this miniprep allows me to run more samples than ever before! I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!"
R.K. CSU
Click here to submit your review of the Direct-zol™ RNA Kits.