For unbiased lysis of microbes and/or solid tissue in TRIzol, use the ZR BashingBead Lysis Tubes (select option) for efficient homogenization of hard-to-lyse samples prior to RNA purification with the Direct-zol RNA kit.  The Direct-zol RNA kit isolates total RNA (including small/miRNAs) from any sample in TRIzol in just 7 minutes.

ZR BashingBead Lysis Tubes (S6003 for animal/plant tissue, S6012 for microbes, S6014 for microbes in tissue/insects) are 2.0 ml screwcap tubes with high density, fracture resistant, chemically inert, ceramic beads and is used upstream for the disruption of hard-to-lyse samples.  Simply collect your sample in TRIzol, transfer the lysate into a Lysis Tube and perform mechanical homogenization (high-speed bench homogenizer adapted for 2.0 ml tubes). Then process the TRIzol lysate with the Direct-zol RNA kit by binding directly to the spin-column, wash and elute.  DNA-free, RNA is ready to use for any downstream application such as NGS, RT/qPCR and etc.