About Zymoprep™-96 Yeast Plasmid Miniprep
The Zymoprep™-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep™-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase™ added in the first solution. There is no need for glass beads, or phenol. Reliably recover your plasmid from yeast cells every time, whether you use colonies, patches on plates, or liquid cultures. The isolated plasmid can be used directly for E. coli transformation, PCR, and Southern blot analysis. The Zymoprep™-96 incorporates our Zymoprep™ II kit into a 96-well format resulting in a high- throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep™ I and eliminates the isopropanol precipitation step . This system is ideal for low- copy number and hard to isolate plasmids and the plasmid DNA can be eluted directly in water or TE buffer.
Two protocols are provided with the Zymoprep™-96 kit. The first protocol is for yeast plasmid recovery from colonies or patches and the second protocol is for liquid culture. Although both protocols work equally well, it is much easier to use colonies or patches to process large numbers of samples.
|DNA Purity||Eluted plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications requiring pure DNA. Abs260/280 is > 1.8.|
|Specificity||S. cerevisiae, C. albicans, S. pombe and other fungi species sensitive to yeast lytic enzymatic digestion (Zymolyase™).|
|Plasmid DNA Size||Up to 25 kb.|
|Plasmid DNA Yield||Typically between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.|
|Procedure||Performed at room temperature (15-30 °C) using a centrifuge with micro plate carriers.|
|Recovery Volume||≥ 10 µl per well.|