- Quick & Easy: Simple 20 minute procedure.
- Highest Yield: Recover 3x more DNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 35 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
|Elution Volume||≥ 35 µl|
|Equipment||Water bath or heat block (55°C), microcentrifuge, and vortex.|
|Purity||High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0).|
|Size Range||Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.|
|Workflow||Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA.|
|Yield||The DNA binding capacity of the column is 25 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
Q1: What is the difference between the digestion buffers? Can one buffer be used for all sample types?
Q2: What is the difference between Quick-DNA and Quick-DNA Plus kits?
Q3: Can Proteinase K digestion be performed overnight in DNA/RNA Shield?
Q4: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
Q5: Can the Quick-DNA Plus kit be used with bacterial samples?
Q6: Can I use Quick-DNA Plus to clean-up previously isolated DNA?
Q7: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Q8: Can Quick-DNA process crude lysates?
This kit is easy to use and provides a high quality yield.
- M.B. (Syracuse University)
“I was very impressed with the purity of the DNA.”
- C.V. (UCLA)
“Improved yield than Bioline and Qiagen Kits.”
- F.C. (Manchester Metropolitan University)
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