RNA purification made simple.
Discover industry-leading RNA purification technology at a fraction of the cost.
RNA Kits Overview
RNA kits play a key role when it comes to obtaining quality RNA from biological samples or reaction mixtures. The kits simplify the process of preserving a sample. RNA kits purify the nucleic acid from the samples and prepare them for downstream applications. Unlike DNA, RNA is extremely sensitive. Therefore, RNA can be a bit trickier to work with due to the presence of ribose and its hydroxyl group. Ribose and its hydroxyl group puts RNA at a much higher risk of hydrolysis.
Nevertheless, many different scientific techniques still require RNA extraction from a sample. That is where RNA analysis kits come into play. RNA analysis kits help to ensure downstream applications are successful by achieving quality RNA. The RNA kits work for many downstream applications: Next-Gen sequencing, real-time quantitative PCR, reverse transcription PCR, microarrays, Northern blot analysis, nuclease protection assays, RNA mapping, in vitro translation, cDNA library construction, etc.
Zymo Research’s RNA kits use methods of extraction to purify or isolate RNA from various types of cells and tissues. It is important to note that they must produce RNA of sufficient yield and structural integrity. That is one of the reasons why it is so critical to ensure that a reliable RNA kit is used. There is a range of RNA extraction kits to ensure efficient recovery of high-quality RNA from a diversity of sources.
What Are RNA Kits For Clean-Up & Concentration?
RNA kits for clean-up and concentration ensure that there is high-quality RNA. High-quality RNA is vital for the success of downstream applications. Examples of some of these downstream applications include: NGS, microarrays, RNA transfection, denaturing-gel electrophoresis, Northern blotting, and RT-PCR. Zymo Research has many different types of RNA kits for clean-up and concentration. All of the RNA kits for clean-up and concentration recover a highly concentrated RNA. When using these kits the structural integrity remains, even in low picogram amounts in less than 6 µl elution volume. Zymo Research’s RNA Clean & Concentrator Kit stands out with the incorporation of its state-of-the-art ‘Fast-Spin’ column technology.
What Is Total RNA Purification?
Zymo Research’s assortment of RNA isolation kits provide the opportunity to achieve simple, rapid, and efficient isolation of total RNA. The RNA isolation kits work for a variety of biological sources including: fresh, frozen, or paraffin-embedded tissues, cultured cells, buccal cells, whole blood, plasma, serum, urine, yeast, or RNA viruses. Similar to RNA clean-up kits, all of the RNA isolation kits feature Fast-Spin column, 96-well plate and magnetic bead-based technology. The innovative technology helps to ensure the recovery of high-quality RNA. The total RNA isolation kits work well with many applications including microarrays, denaturing-gel electrophoresis, Northern blotting, and RT-PCR.
All of Zymo Research’s kits are available in a variety of formats. These formats include Micro-, Mini-, Midi- and 96-well format based on binding capacities. There are also supplemental protocols available to isolate specific RNA species such as total RNA (>17 nt), large RNAs (>200 nt) and small/micro RNAs (200-17 nt).
Zymo Research has many RNA kits with specialty components. These specialty components are critical to ensure key factors. These key factors include maximum levels of membrane solubilization and cellular disruption, total inhibition of nuclease activity, complete deproteinization of the sample, efficient isolation and concentration of RNA, and stabilization and storage of the RNA. All of these items are essential when working with smaller RNA species. Zymo Research’s RNA kits help simplify the entire purification process for small RNA species.
What's Next For RNA Kits?
Zymo Research is paving the way for the future of RNA kits with new innovative technology. Currently, Zymo Research has RNA kits and DNA/RNA extraction kits such as the Quick-RNA™, Direct-zol™ RNA, and Quick-DNA/RNA™ Kits. Zymo Research also has specialty kits for viral isolation and high-throughput extraction of nucleic acids. The majority of these RNA and DNA/RNA kits have different base options to extract high-quality nucleic acids for real-world applications. RNA and DNA/RNA kits are available with different bases. The bases available include column-based technology or high-throughput, and magnetic bead-based technology for robotic platforms and automation.
Groundbreaking innovations such as the Direct-zol™ RNA Kits have made it easy and quick to achieve higher RNA yields. The superior quality of RNA yields works especially well with samples in TRIzol®. Now extracting RNA from TRIzol® can occur in only seven minutes. There is no longer a need for phase separation, precipitation, or post-purification steps. The procedure is simple and easy and only takes three steps: bind, wash and elute. Samples in TRIzol® can go directly to the Zymo-Spin Column and then bind, wash, and elute total RNA including small/microRNAs. The RNA is then ready for any downstream application.
On the other hand, the Quick-RNA™ kits use a non-organic-based RNA extraction method. This type of method offers universal sample compatibility. The universal sample compatibility is suitable for any input such as cells, tissue, biological samples, and microbes. The technology is one-of-a-kind. It is NGS-ready and offers total RNA including small/micro-RNA recovery, a DNA removal column and DNase I. It also provides consistent high-quality RNA. The high-quality RNA is NGS-ready and works for a variety of applications including: RNA-seq and library preparation, RT-PCR & RT-qPCR, northern blot analysis, and microarray analysis.