Direct-zol DNA/RNA Miniprep

R2080T / R2080 / R2081


Direct-zol DNA/RNA Miniprep

R2080T / R2080 / R2081

Cat # Name Size Price Quantity
R2080T Direct-zol DNA/RNA Miniprep 10 preps $79.00
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R2080 Direct-zol DNA/RNA Miniprep 50 preps $229.00
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R2081 Direct-zol DNA/RNA Miniprep (Product Supplied with TRI Reagent) 50 preps $293.00
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Documents


Direct-zol DNA/RNA Miniprep kit

Highlights


  • One Input, One Column: Purify RNA and DNA into separate fractions with just one spin-column, directly from a sample in TRIzol, TRI Reagent® or a similar acid-guanidinium-phenol based reagent.
  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • High-Quality: DNA, RNA (including small/miRNAs) is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.

Description


The Direct-zol DNA/RNA kits provide an innovative method for the purification of DNA and total RNA from a variety of samples freshly lysed in TRIzol or similar, including animal cells, tissue, bacteria, yeast, plant, biological liquids and etc.

Upon lysis of the sample with TRIzol or similar, RNA and DNA is bound directly to the Zymo-Spin Column. Then simply spin, wash, and elute high-quality RNA and DNA into separate fractions. No phase separation, precipitation, or post-purification steps are necessary. The eluted nucleic acids are suitable for all subsequent molecular manipulations and analyses including Next-Gen sequencing, RT/qPCR, hybridization, etc.

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Technical Specifications


Binding Capacity Specification 25 µg DNA and 50 µg RNA (yield will vary with type and treatment of sample)
Compatibility Aqueous (RNA) phase from TRIzol, TRI Reagent® or similar can be processed. Also, compatible with samples in DNA/RNA Shield (see Appendix, page 5)
Elution Volume ≥ 25 µl
Equipment Microcentrifuge
Purity DNA and RNA ready for all subsequent downstream analyses (Next-Gen sequencing, RT/qPCR)
Sample Inactivation TRI Reagent® (provided with R2081, R2083 only) inactivates viruses and other infectious agents.
Sample Type Specifications Cells (animal, bacterial, yeast), tissue (animal, plant) or biological liquids (blood, plasma, serum, CSF, buffy coat, etc.), freshly lysed in TRIzol, TRI Reagent® or similar.
Size Range up to and above 40 kb (DNA), ≥ 17 nt (RNA)

Kit Components