E. coli Transformation Made Simple
Transformation in 20 Seconds
Free Samples Available
A Sweet Solution to Obesity
A New Era of Effortless Cloning
E. coli is the most extensively studied model organism in molecular biology. Despite their widespread use in laboratories across the world for applications such as cloning and protein expression, protocols for manipulating and studying E. coli tend to be long and tedious. With speed and simplicity as the driving principles, we embarked on a mission to create a new and improved molecular cloning system.
Our novel chemical shock technology eliminates heat shock and outgrowth procedures, cutting down hours of processing time. Today, our Mix & Go! chemical shock method is available in five pre-made E. coli strains and can be applied to nearly any other lab strain, with high transformation efficiencies of up to 109 transformants/µg plasmid DNA. For cloning beginners and veterans alike, Mix & Go! offers a welcome relief from the hassles of traditional bacterial transformation.
Knowledge Center
Solutions For E. coli
Testimonials from Our Customers
“ A true time saver. This product has saved me countless hours in addition to yielding more viable post-transformation colonies than any other equivalent products I have tested in the past. ”
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California Institute of Technology
“ Makes DNA cloning much easier! It is a much less involved process than traditional competent cells, and only took me less than a day's time to make enough cells to last our lab at least 6 months. ”
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Cornell University
“ Effective and a huge time saver! High quality reproducible results, very quick and easy compared to traditional transformation. ”
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University of Pittsburgh
“ Inexpensive, easy to use and reliable competent cells. The price is very reasonable and made it easy to change from using homemade competent cells to using commercially made. This saves us time and energy and has vastly increased the reliability of our work. ”
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Missouri University of Science and Technology
“ Plenty of colonies and wicked fast protocol! These are now my go to. ”
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Massachusetts General Hospital
“ Good price compared to store bought competent cells, very easy and quick to use, excellent alternative to normal cloning & transformation especially when time needed for normal incubations associated with transformations isn't available. ”
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Rowan University
Promotions
Our Gift to You! Happy Holidays from Zymo
This December, Zymo Research is celebrating 30 years of innovation & passion with special promotions for our valued customers!
- Holiday Gift with Purchase: Receive a complimentary Holiday Gift Pack, including a $10 Starbucks gift card and a festive bacteria-shaped cookie cutter, with any purchase! Simply opt in to promo code ZYMO-GIFT in your cart.
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Complete Your NGS Workflow Offer: Choose products from our Sample Collection, DNA or RNA Purification, and NGS Library Prep categories and use promo code ZYMO-JOY to receive these exclusive offers:
- 20% OFF when you purchase eligible products from any two categories.
- 30% OFF when you purchase eligible products from all three categories.
Explore our DNA and RNA Workflows
Featured Technologies
Strain Selection Guides
Propagation Strains
Ideal for cloning, plasmid isolation, and library construction
| JM109 | DH5 Alpha | HB101 | TG1 | Zymo 10B | |
|---|---|---|---|---|---|
| Specifications | |||||
| Strain Background | K-12 | K-12 | K-12 | K-12 | K-12 |
| General Cloning | ✓ | ✓ | ✓ | ✓ | ✓ |
| Plasmid Isolation | ✓ | ✓ | ✓ | ✓ | ✓ |
| Recombinant Protein Expression | ✓ | ||||
| Production of ssDNA (F' episome) | ✓ | ✓ | |||
| Suppression of Amber Mutations (glnV44 or supE44) | ✓ | ✓ | ✓ | ✓ | |
| Blue-White Selection (lacZΔM15) | ✓ | ✓ | ✓ | ✓ | |
| High-quality and Yield of Plasmid DNA (endA1) | ✓ | ✓ | ✓ | ||
| Reduced Recombination & Insert Stability (recA1 or recA13) | ✓ | ✓ | ✓ | ✓ | |
| Plasmid Size | Up to 10-15 kb | Up to 10-15 kb | Up to 10-15 kb | ||
| Transformation of Large Plasmids (deoR) | Up to 20-32 kb | Up to 20-32 kb | |||
| Ampicillin Resistant (bla or ampR) | |||||
| Chloramphenicol Resistant (cat or CmR or CamR) | |||||
| Tetracycline Resistant (Tn10 or tetR) | |||||
| Kanamycin Resistant (KanR) | |||||
| Nalidixic Acid Resistant (gyrA96 or NalR) | ✓ | ✓ | |||
| Streptomycin Resistant (StrR) | ✓ | ✓ | |||
| Genotype | F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1 | F- φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1, λ- | F- Δ(gpt-proA)62 leuB6 glnV44 (supE44) ara-14 galK2 lacY1 Δ(mcrC-mrr) xyl-5 mtl-1 recA13 thi-1 rpsL20 (SmR) | F’[traD36 lacIq Δ(lacZ) M15 proA+B+] glnV (supE) thi-1 Δ(mcrB-hsdSM)5 (rK- mK- McrB-) thi Δ(lac-proAB) | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- |
| Catalog Number | T3003 | T3007 | T3011 | T3017 | T3019 |
Expression Strains
Ideal for protein expression and DNA analysis
| XJa Autolysis™ |
XJa (DE3) Autolysis™ |
XJb Autolysis™ | XJb (DE3) Autolysis™ |
|
|---|---|---|---|---|
| Specifications | ||||
| Strain Background | K-12 | K-12 | B | B |
| General Cloning | ✓ | ✓ | ||
| Plasmid Isolation | ✓ | ✓ | ||
| General Screening | ✓ | ✓ | ||
| Recombinant Protein Expression | ✓ | ✓ | ✓ | ✓ |
| Production of ssDNA (F' episome) | ✓ | ✓ | ||
| T7 Promoter Transcription (λDE3) | ✓ | ✓ | ||
| Autolysis (ΔaraB::λR) | Autolysis Inducible by Arabinose |
Autolysis Inducible by Arabinose |
Autolysis Inducible by Arabinose |
Autolysis Inducible by Arabinose |
| Suppression of Amber Mutations (glnV44 or supE44) | ✓ | ✓ | ||
| Blue-White Selection (lacZΔM15) | ✓ | ✓ | ||
| High-quality and Yield of Plasmid DNA (endA1) | ✓ | ✓ | ||
| Reduced Recombination & Insert Stability (recA1 or recA13) | ✓ | ✓ | ||
| Plasmid Size | Up to 10 kb | Up to 10 kb | Up to 10 kb | Up to 10 kb |
| Transformation of Large Plasmids (deoR) | ||||
| Ampicillin Resistant (bla or ampR) | ||||
| Chloramphenicol Resistant (cat or CmR or CamR) | ✓ | ✓ | ✓ | ✓ |
| Tetracycline Resistant (Tn10 or tetR) | ||||
| Kanamycin Resistant (KanR) | ||||
| Nalidixic Acid Resistant (gyrA96 or NalR) | ||||
| Streptomycin Resistant (StrR) | ||||
| Genotype | F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR) | F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44)e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR), λ(DE3) | F- ompT hsdSB(rB- mB-) gal dcm ΔaraB::λR, cat (CmR) | F- ompT hsdSB(rB- mB-) gal dcm ΔaraB::λR, cat (CmR), λ(DE3) |
| Catalog Number | T3021/T5021 | T3031/T5031 | T3041/T5041 | T3051/T5051 |
Custom & OEM Solutions
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