- Quick recovery of ultra-pure DNA from agarose gels.
- Column design permits DNA elution at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
|Applicable For||Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.|
|Elution Volume||≥ 6 µl of DNA Elution Buffer|
|Purity||A260/A280 >1.8, A260/A230>1.8|
|Sample Source||DNA excised from TAE/TBE-buffered agarose gels.|
|Size Range||50 bp to 23 kb|
|Yield||Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%|
Q1: Is it possible to use higher binding capacity columns for the Zymoclean workflow?
Q2: Are the columns and DNA Wash buffer interchangeable with the DCC-5 Kit?
Q3: Should more Agarose Dissolving Buffer be used for higher % gels?
Q4: Can Zymoclean be used for samples in solution (without agarose gel)?
Q5: Can Zymoclean be used to recover ssDNA?
Q6: What is the maximum weight of gel slice that can be used?
Anaerobic bacterial strains from Antartica were sequenced for phylogenetic analysis and molecular studies revealed the possibility of a novel genus and species using 16S rRNA sequence and the recA gene. The Zymoclean Gel DNA Recovery Kit was used to gel purify recA PCR bands to ensure successful amplification.Lyu Z, et. al. (2015). Molecular studies of anaerobic strains from Antarctica and their taxonomic identification. SPIE Proceedings.
The Zymoclean Gel DNA Recovery Kit was used to purify Cytochrome P450 (CYP) encoding PCR products and was followed by cloning in a yeast expression vector for CYP production. Researchers reported new CYPs involved in the synthesis of phytoalexins, antimicrobial toxins against plant pathogens.Klein AP, et. al. (2015). Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis. Nature Chemical Biology.
The Zymoclean Gel DNA Recovery Kit was used to recover PCR products, which was then used in high-throughput sequencing to elucidate epigenetic variables and sequences to improve CRISPR-Cas9 genome targeting and editing. An in-vivo library-on-library approach was utilized for the simultaneous assessment of single guide RNA activity across 1,400 genes from various bacterial species.Chari R, et. al. (2015). Unraveling CRISPR-Cas9 genomic engineering parameters via a library-on-library approach. Nature Methods.
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
- Ingrid M.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
- Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
- Simin H. (University of California, Irvine)