Mix & Go! Competent Cells-TG1

T3017


Mix & Go! Competent Cells-TG1

T3017

Cat # Name Size Price Quantity
T3017 Mix & Go! Competent Cells-TG1 10 x 100 µl $129.00
+ -

Documents


Mix & Go! Competent Cells-TG1

Highlights


  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.

Description


The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go!E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical Specifications


Additional Info Can be used for general cloning and blue/white screening.
Genotype F’[traD36 lacIq Δ(lacZ) M15 proA+B+] glnV (supE) thi-1 Δ(mcrB-hsdSM)5 (rK- mK- McrB-) thi Δ(lac-proAB)
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA

Product FAQ


Q1: Which antibiotics can be used with the Mix & Go! procedure?

Q2: How will a heat-shock affect my Transformation Efficiency?

Q3: Are competent cells GMOs?

Q4: Are the Mix & Go! strains dam+ and dcm+?

Q5: Do the Mix & Go! strains methylate DNA?

Q6: Which strains are equivalent to the Zymo strains?

Q7: How to reduce satellite colonies on agar plates?

Q8: Is it possible to dilute the competent cells?

Q9: What are some tips to improve transformation efficiency?

Q10: Which Plasmid Size can be used for transformation?

Q11: Which is the recommended DNA concentration and volume for transformation?

Product Video