Mix & Go! Competent Cells -JM109

T3003 / T3005


Mix & Go! Competent Cells -JM109

T3003 / T3005

Cat # Name Size Price Quantity
T3003 Mix and Go! Competent Cells -JM109 10 x 100 µl $129.00
+ -
T3005 Mix and Go! Competent Cells -JM109 96 x 50 µl $494.00
+ -

Documents


For simple, high efficiency transformations without heat shock and lengthy incubations.

Highlights


  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.

Description


The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical Specifications


Additional Info Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening.
Genotype F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA

Product FAQ


Q1: Are competent cells GMOs?

Q2: Are the Mix & Go! strains dam+ and dcm+?

Q3: Do the Mix & Go! strains methylate DNA?

Q4: Which strains are equivalent to the Zymo strains?

Q5: How to reduce satellite colonies on agar plates?

Q6: Is it possible to dilute the competent cells?

Q7: Which antibiotics can be used with the Mix & Go! procedure?

Q8: Which Plasmid Size can be used for transformation?

Q9: Which is the recommended DNA concentration and volume for transformation?

Q10: What are some tips to improve transformation efficiency?

Q11: How will a heat-shock affect my Transformation Efficiency?

Product Video


Citations


The authors found that the narrow-spectrum mercury resistance (mer) operon of Tenacibaculum discolor encode a novel ArsR/SmtB family transcriptional regulator that controls the expression of a mercuric reductase. To confirm that the ArsR/SmtB transcriptional regulator was mercury-responsive, a reporter plasmid was constructed by replacing the reductase gene with a luciferase gene and transformed into Mix & Go Competent E. coli cells, strain JM109, from Zymo Research. The purified plasmid was used to carry out a functional assay that showed the regulator can be stimulated by low concentrations of mercury.

Allen, R.C. et al. (2013) “The mercury resitance (mer) operon in a marine gliding flavobacterium, Tenacibaculum discolor 9A5.” FEMS Microbiol Ecol 83:135-148.