Mix & Go! Competent Cells -JM109
T3003 / T3005
- Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
- High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
- Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
|Additional Info||Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening.|
|Genotype||F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1|
|Processing Time||20 Seconds|
|Product Storage||-70°C to -80°C|
|Transformation Efficiency||108 - 109 transformants per µg of plasmid DNA|
Q1: Are competent cells GMOs?
Q2: Are the Mix & Go! strains dam+ and dcm+?
Q3: Do the Mix & Go! strains methylate DNA?
Q4: Which strains are equivalent to the Zymo strains?
Q5: How to reduce satellite colonies on agar plates?
Q6: Is it possible to dilute the competent cells?
Q7: Which antibiotics can be used with the Mix & Go! procedure?
Q8: Which Plasmid Size can be used for transformation?
Q9: Which is the recommended DNA concentration and volume for transformation?
Q10: What are some tips to improve transformation efficiency?
Q11: How will a heat-shock affect my Transformation Efficiency?
The authors found that the narrow-spectrum mercury resistance (mer) operon of Tenacibaculum discolor encode a novel ArsR/SmtB family transcriptional regulator that controls the expression of a mercuric reductase. To confirm that the ArsR/SmtB transcriptional regulator was mercury-responsive, a reporter plasmid was constructed by replacing the reductase gene with a luciferase gene and transformed into Mix & Go Competent E. coli cells, strain JM109, from Zymo Research. The purified plasmid was used to carry out a functional assay that showed the regulator can be stimulated by low concentrations of mercury.Allen, R.C. et al. (2013) “The mercury resitance (mer) operon in a marine gliding flavobacterium, Tenacibaculum discolor 9A5.” FEMS Microbiol Ecol 83:135-148.