Mix & Go! Competent Cells -JM109
T3003 / T3005
- Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
- High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
- Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
|Additional Info||Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g.,λgt11. Can be used for M13 cloning/sequencing and blue/white screening.|
|Genotype||F`[traD36 proA+B+ laclq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1|
|Processing Time||20 Seconds|
|Product Storage||-70°C to -80°C|
|Transformation Efficiency||108 - 109 transformants per µg of plasmid DNA|
Q1: Are competent cells GMOs?
Q2: Are the Mix & Go! strains dam+ and dcm+?
Q3: Do the Mix & Go! strains methylate DNA?
Q4: Which strains are equivalent to the Zymo strains?
Q5: How to reduce satellite colonies on agar plates?
Q6: Is it possible to dilute the competent cells?
Q7: Which antibiotics can be used with the Mix & Go! procedure?
Q8: Which Plasmid Size can be used for transformation?
Q9: Which is the recommended DNA concentration and volume for transformation?
Q10: What are some tips to improve transformation efficiency?
Q11: How will a heat-shock affect my Transformation Efficiency?
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