Mix & Go! E.coli Transformation Kit and Buffer Set

T3001 / T3002


Mix & Go! E.coli Transformation Kit and Buffer Set

T3001 / T3002

Cat # Name Size Price Quantity
T3001 Mix & Go! E.coli Transformation Kit up to 20 ml $114.00
+ -
T3002 Mix & Go! E.coli Transformation Buffer Set (Zymobroth not included) up to 60 ml $125.00
+ -

Documents


Reagents to prepare Mix & Go Competent Cells from E. coli strains

Highlights


  • Easy 3 Step Protocol: Produce reliable chemically competent E. coli in less than 45 minutes.
  • Simple 20 sec Transformation: No heat shock! Just add DNA and spread.
  • High Transformation Efficiencies: Achieve 108-109 transformants per µg of plasmid DNA.

Description


The Mix & Go! E. coli Transformation Kit and Mix & Go! E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go! method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go! bacterial transformation can be performed by adding DNA to Mix & Go! competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/µg plasmid DNA with most E. coli strains. Uniquely formulated reagents make it easy to generate Mix & Go! competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go! E. coli Transformation Kit includes all buffers and ZymoBroth medium to generate 20 ml of Mix & Go! competent cells. The Mix & Go! E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go! competent cells, and the medium (broth) is supplied by the user.

Technical Specifications


Compatible Strains The Kit is optimized to work with K12 and B derivative strains. Some commonly used strains include, TOP10, ccdB survival, XL1 Blue and Stbl3.
Processing Time ≤ 45 minutes
Product Storage Please store the Wash Buffer (2X), Competent Buffer (2X), and Dilution Buffer at 0-8°C. The ZymoBroth can be stored at room temperature.
Transformation Efficiency 108-109 transformants per µg of plasmid DNA for most common lab strains.

Product FAQ


Q1: Which E. coli strains can be used with the Transformation Kit?

Q2: Are there any specific considerations in preparing of overnight culture?

Q3: How long can the cells sit in 1X Wash buffer?

Q4: How long can the cells sit in 1X Competent buffer?

Q5: How can I boost transformation efficiencies?

Q6: I accidentally stored the kit at room temperature. Is it still okay to use?

Q7: What are the best practices for aliquoting the competent cells?

Q8: Can competent cells be stored in liquid nitrogen (N2)?

Q9: Is the transformation efficiency of 108 -109 guaranteed for the Mix & Go! E..coli Transformation Kit & Buffer Set.

Citations


Z-competent DH5α E. coli cells were prepared using the Mix & Go E. coli Transformation Buffer Set from Zymo Research and used for high-efficiency transformations in a study that tested the strength and compatibility of varying transcription terminators, which often need to be used in series to stop transcription originating from the strong promoters required in synthetic circuits. Data from this study highlighted 39 terminators that not only have the ability to reduce downstream expression by >50-fold, but also have enough sequence diversity to be used together.

Chen, Y. et al. (2013) Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature Methods 10 (2013): 659-664.

To determine the structural requirements for a catalytically active histidine triad nucleotide protein (ecHinT) that allows Escherichia coli to grow on D-alanine as a sole carbon source, the authors designed various plasmids that encoded mutant Hint proteins. Competent HinT-deficient E. coli were prepared using the Mix & Go E. coli Transformation Kit from Zymo Research and were then transformed using the designed plasmids. The authors found that the C-terminal loop of ecHinT is an important structure of an active ecHinT.

Bardaweel, S. et al. (2011) E. coli histidine triad nucleotide binding protein 1 (ecHinT) is a catalytic regulator of D-alanine dehydrogenase (DadA)

Kit Components