DNA Clean & Concentrator-5

D4004 / D4003 / D4014 / D4013 / D4003T


DNA Clean & Concentrator-5

D4004 / D4003 / D4014 / D4013 / D4003T

Cat # Name Size Price Quantity
D4004 DNA Clean & Concentrator-5 (Uncapped) 200 Preps $272.00
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D4003 DNA Clean & Concentrator-5 (Uncapped) 50 Preps $75.00
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D4014 DNA Clean & Concentrator-5 (Capped) 200 Preps $272.00
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D4013 DNA Clean & Concentrator-5 (Capped) 50 Preps $75.00
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D4003T DNA Clean & Concentrator-5 (Uncapped) 10 Preps $25.00
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Documents


Clean-up PCR and other enzymatic reactions in as little as 2 minutes

Highlights


  • Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
  • Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Description


The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.

Technical Specifications


Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume ≥ 6 µl
Equipment Microcentrifuge
Purity A260/A280 > 1.8
Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range 50 bp to 23 kb
Yield ≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Product FAQ


Q1: What is the difference between capped and uncapped?

Q2: What is the minimum input volume of DNA sample?

Q3: How many times can columns be reloaded?

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Q5: How can I process naked DNA stored in DNA/RNA Shield?

Q6: What is the lower limit and minimal amount of DNA that can be recovered?

Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?

Product Video


Citations


PCR products were purified using the DNA Clean & Concentrator-5 for CRISPR sgRNA oligo synthesis and then used as template for in vitro transcription. This reverse genetic screening strategy, which incorporated Cas9-encoding mRNA in zebrafish, was used to examine 48 loci, resulting in the identification of two new genes involved in electrical-synapse formation by deep sequencing.

Shah AN, et. al. (2015). Rapid reverse genetic screening using CRISPR in zebrafish. Nature Methods. 12(6): 535-40

Genomic DNA from P. obducens, the causal agent of downy mildew disease, was crudely extracted and then purified using the DNA Clean & Concentrator-5. Simple sequence repeat (SSR) markers were developed, which can be used to further assess variation in pathogen population and factors of disease emergence.

Salgado-Salazar C, et. al. (2015). Polymorphic SSR Markers for Plasmopara obducens (Peronosporaceae), the Newly Emergent Downy Mildew Pathogen of Impatiens(Balsaminaceae) Applications in Plant Sciences. 3(11).

DNA from anaerobic microbial strains was extracted from meteorites and the PCR products were purified using the DNA Clean & Concentrator-5. Phylogenetic analysis by 16S rRNA sequencing showed common bacterial genera including Bacillus and Clostridium, which may indicate contamination from modern microbes in soil.

Pikuta EV, et. al. (2015).Microbiological investigation of two chondrite meteorites: Murchison and Polonnaruwa. SPIE Proceedings.

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Reviews


“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”

- Shanshan L. (UT Southwestern)

"I had low-volume, low-concentration, high-molecular weight genomic DNAs for high-throughput sequencing that I could not get to be perfectly clean without losing a significant amount of my (precious) sample. The Qiagen products were recommended by the company doing the sequencing, and they just ate up my sample. When I received this sample with my mini-prep kit, I was at the end of the line and figured I'd give it a try, despite being warned against anything involving spin columns that may shear the DNA. It worked beautifully. We can proceed with our project. No loss of sample, the gel looks the same (no sign of DNA shearing), and what an easy and fast protocol! Thank you, Zymo!"

- Cindy T. (University of Iowa)

“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”

- Rosalind P. (University of Arkansas for Medical Sciences)

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Kit Components