Mix & Go! Competent Cells-Zymo 10B

T3019 / T3020


Mix & Go! Competent Cells-Zymo 10B

T3019 / T3020

Cat # Name Size Price Quantity
T3019 Mix & Go! Competent Cells-Zymo 10B 10 x 100 µl $129.00
+ -
T3020 Mix & Go! Competent Cells-Zymo 10B 96 x 50 µl $494.00
+ -

Documents


For simple, high efficiency transformations without heat shock and lengthy incubations.

Highlights


  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.

Description


The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical Specifications


Additional Info Can be used for blue/white screening and is ideal for cDNA generation and library construction.
Genotype F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ-
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA

Product FAQ


Q1: Which Plasmid Size can be used for transformation?

Q2: Which is the recommended DNA concentration and volume for transformation?

Q3: Which antibiotics can be used with the Mix & Go! procedure?

Q4: Is it possible to dilute the competent cells?

Q5: How to reduce satellite colonies on agar plates?

Q6: Which strains are equivalent to the Zymo strains?

Q7: How will a heat-shock affect my Transformation Efficiency?

Q8: Are the Mix & Go! strains dam+ and dcm+?

Q9: Are competent cells GMOs?

Q10: What are some tips to improve transformation efficiency?

Q11: Do the Mix & Go! strains methylate DNA?

Product Video