Mix & Go! Competent Cells - DH5 Alpha

T3007 / T3009 / T3010


Mix & Go! Competent Cells - DH5 Alpha

T3007 / T3009 / T3010

Cat # Name Size Price Quantity
T3007 Mix & Go! Competent Cells - DH5 Alpha 10 x 100 µl $129.00
+ -
T3009 Mix & Go! Competent Cells - DH5 Alpha 96 x 50 µl $494.00
+ -
T3010 Mix & Go! Competent Cells - DH5 Alpha w/ 96-well PCR plates and Cover Foils 96 x 50 µl $494.00
+ -

Documents


For simple, high efficiency transformations without heat shock and lengthy incubations.

Highlights


  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.

Description


The Mix & Go! Competent Cells - DH5 Alpha are premade E. coli DH5 alpha competent cells for simple and highly efficient DNA transformation. These DH5 alpha competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical Specifications


Additional Info Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.
Genotype F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ-
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA

Product FAQ


Q1: Are competent cells GMOs?

Q2: Are the Mix & Go! strains dam+ and dcm+?

Q3: Do the Mix & Go! strains methylate DNA?

Q4: Which strains are equivalent to the Zymo strains?

Q5: How to reduce satellite colonies on agar plates?

Q6: Is it possible to dilute the competent cells?

Q7: Which antibiotics can be used with the Mix & Go! procedure?

Q8: Which Plasmid Size can be used for transformation?

Q9: Which is the recommended DNA concentration and volume for transformation?

Q10: What are some tips to improve transformation efficiency?

Q11: How will a heat-shock affect my Transformation Efficiency?

Product Video


Citations


Reviews


“It saves time and money and it really works! I chose this kit because it was regularly used by the people at Addgene.com. It is really easy to make and the transfection efficiency is higher than what I would get from the homemade competent cells. The feature that I like the most about this kit is that it takes less than 5 min to perform the whole transformation procedure (with ampicillin as a selection marker) instead of a regular 1.30 H!”

- Cho R. (Michigan State University)

“Worth the price. Great transformation efficiency, save so much time from the traditional transformation protocol, awesome price and last forever. Thanks!”

- Yu L. (Caltech)

“The fastest competent cells. Revolutionary! No need to care for the delicate transfection of your E.coli, Mix&Go has shortened the process from 2h to 5min. Simply add a few microliters of your plasmid on ice, wait 5min, Voila!"

- Jerome B (UCSF)