- Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
- High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
- Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
|Additional Info||Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.|
|Genotype||F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ-|
|Processing Time||20 Seconds|
|Product Storage||-70°C to -80°C|
|Transformation Efficiency||108 - 109 transformants per µg of plasmid DNA|
Q1: Are competent cells GMOs?
Q2: Are the Mix & Go! strains dam+ and dcm+?
Q3: Do the Mix & Go! strains methylate DNA?
Q4: Which strains are equivalent to the Zymo strains?
Q5: How to reduce satellite colonies on agar plates?
Q6: Is it possible to dilute the competent cells?
Q7: Which antibiotics can be used with the Mix & Go! procedure?
Q8: Which Plasmid Size can be used for transformation?
Q9: Which is the recommended DNA concentration and volume for transformation?
Q10: What are some tips to improve transformation efficiency?
Q11: How will a heat-shock affect my Transformation Efficiency?
“It saves time and money and it really works! I chose this kit because it was regularly used by the people at Addgene.com. It is really easy to make and the transfection efficiency is higher than what I would get from the homemade competent cells. The feature that I like the most about this kit is that it takes less than 5 min to perform the whole transformation procedure (with ampicillin as a selection marker) instead of a regular 1.30 H!”
- Cho R. (Michigan State University)
“Worth the price. Great transformation efficiency, save so much time from the traditional transformation protocol, awesome price and last forever. Thanks!”
- Yu L. (Caltech)
“The fastest competent cells. Revolutionary! No need to care for the delicate transfection of your E.coli, Mix&Go has shortened the process from 2h to 5min. Simply add a few microliters of your plasmid on ice, wait 5min, Voila!"
- Jerome B (UCSF)
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