Mix & Go! Competent Cells - DH5 Alpha

T3007 / T3009 / T3010


Mix & Go! Competent Cells - DH5 Alpha

T3007 / T3009 / T3010

Cat # Name Size Price Quantity
T3007 Mix & Go! Competent Cells - DH5 Alpha 10 x 100 µl $129.00
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T3009 Mix & Go! Competent Cells - DH5 Alpha 96 x 50 µl $494.00
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T3010 Mix & Go! Competent Cells - DH5 Alpha w/ 96-well PCR plates and Cover Foils 96 x 50 µl $494.00
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Documents


Data Sheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
For simple, high efficiency transformations without heat shock and lengthy incubations.

Highlights


  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.

Description


The Mix & Go! Competent Cells - DH5 Alpha are premade E. coli DH5 alpha competent cells for simple and highly efficient DNA transformation. These DH5 alpha chemically competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical Specifications


Additional Info Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.
Genotype F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ-
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA

Product FAQ


Q1: Are competent cells GMOs?

Q2: Are the Mix & Go! strains dam+ and dcm+?

Q3: Do the Mix & Go! strains methylate DNA?

Q4: Which strains are equivalent to the Zymo strains?

Q5: How to reduce satellite colonies on agar plates?

Q6: Is it possible to dilute the competent cells?

Q7: Which antibiotics can be used with the Mix & Go! procedure?

Q8: Which Plasmid Size can be used for transformation?

Q9: Which is the recommended DNA concentration and volume for transformation?

Q10: What are some tips to improve transformation efficiency?

Q11: How will a heat-shock affect my Transformation Efficiency?

Citations


The mechanisms of electrotransfection of mammalian cells were determined by observing the interaction of plasmid DNA (pDNA) with the cell membrane and internalization of the pDNA into the cell. The plasmid used in the study was amplified using Mix & Go E. coli Competent Cells, strain Zymo 5α, from Zymo Research, and labeled with a fluorescence dye to visualize pDNA binding and internalization. The authors found that efficient electrotransfection is dependent on the presence of divalent cations and endocytic pathways that can transport the pDNA across the membrane.

Wu, M. and F. Yuan. (2011) Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cell. PloS One 6:e20923.

In the process of developing a protocol to facilitate real-time imaging of apoptotic cell membrane changes in live cells, researchers used the Mix & Go Z-competent E.coli strain Zymo 5α from Zymo Research for fast, high-efficiency transformations to express annexin B12 modified with fluorophores that fluoresce when bound to phosphatidylserine-containing membranes.

Kim, Y.E. et al. (2010) Monitoring apoptosis and neuronal degeneration by real-time detection of phosphatidylserine externalization using a polarity-sensitive indicator of viability and apoptosis. Nature Protocols 5: 1396-1405.