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    Quick-RNA Fecal/Soil Microbe Microprep Kit

    The Quick-RNA Fecal/Soil Microbe Microprep Kit is an affordable fecal and soil RNA extraction kit designed for fast, easy isolation of total RNA including small RNAs from soil, sludge, sediment and fecal samples.

    Highlights

    • High Quality: Extract intact high quality total RNA (including small/micro RNAs) from various soil, fecal, and water samples.
    • Homogenization: Ultra-high density BashingBead technology for complete disruption of tough-to-lyse samples.
    • Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Highlights

    • High Quality: Extract intact high quality total RNA (including small/micro RNAs) from various soil, fecal, and water samples.
    • Homogenization: Ultra-high density BashingBead technology for complete disruption of tough-to-lyse samples.
    • Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size Price Quantity
    R2040 Quick-RNA Fecal/Soil Microbe Microprep Kit 50 preps $402.60
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    Cat # Name Size Price
    W1001-6 DNase/RNase-Free Water 6 ml $17.50
    C1004-50 Zymo-Spin IC Columns 50 Pack $61.80
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack $64.20
    R1060-2-25 RNA Prep Buffer 25 ml $46.70
    R2040-1-50 S/F RNA Lysis Buffer 50 ml $122.60
    R1013-2-50 RNA Binding Buffer 50 ml $87.60
    R1003-3-24 RNA Wash Buffer 24 ml $68.80
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes $117.80
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack $126.10

    Description

    The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from various soil, sludge, sediment and/or fecal samples. The procedure of this fecal and soil RNA extraction kit successfully isolates RNA from tough-to-lyse bacteria, fungi, protozoa (protist), algae, etc. in soil, and host RNA from fecal samples. Samples are added to the ZR BashingBead Lysis Tube with an optimally designed S/F RNA Lysis Buffer where microbes are then lysed by bead beating to extract total RNA. The Zymo-Spin column technology included in this fecal and soil RNA extraction kit allows for quick filtration, genomic DNA removal from sample lysates, and isolation of the RNA. Zymo-Spin III-HRC Spin Filter separates RT-PCR inhibitors (e.g., humic acids, polyphenols, tannins) and the total RNA is concentrated using the Zymo-Spin IC Column with a minimum elution volume of ≥6 µl.

    Performance

    Technical Specifications
    Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Bacteria, fungi, protozoa, and algae in up to 250 mg soil, sludge or sediments, and bacteria, protist and/or host RNA from feces (mammalian, avian, etc.)
    Size Range Total RNA ≥ 17 nt
    Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

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