Bisulfite Conversion Kits

Choose from the best bisulfite conversion kits selection available in spin-column, 96-well, and MagBead formats. These innovative bisulfite conversion kits feature streamlined workflows, ≥ 99% conversion rates, automation friendly options, and low elution volumes for concentrated, bisulfite-converted DNA.

EZ DNA Methylation-Lightning Kit EZ DNA Methylation-Direct Kit EZ DNA Methylation EZ DNA Methylation-Gold
Extremely fast and simple to use Use directly with cells & tissues! Recommended for Illumina Infinium Arrays Classic bisulfite conversion
1.5 hr 4 hr 12 - 16 hr 3 hr
> 99.5% > 99.5% > 99% > 99%
Input 100 pg - 2 µg of DNA DNA(> 50 pg), cells (> 10), blood, tissue, FFPE 500 pg - 2 µg of DNA 500 pg - 2 µg of DNA
Validated For Roche NimbleGen SeqCap Epi Accel-NGS Adaptase for Single Cell Methyl-Seq Illumina Infinium Methylation EPIC Array / 450K Array Agilent SureSelect MethylSeq, Swift Accel-NGS Methyl-Seq
Product Name
Overview - Bisulfite Conversion Kits

Sodium bisulfite conversion of DNA is the fastest, easiest, and most popular way to study DNA methylation analysis and selecting the right bisulfite conversion kit can be the difference between accurate data and frustrating wasted hours spent in the lab. Choosing a bisulfite conversion kit is an important step in DNA methylation studies to ensure that you obtain the highest quality bisulfite-converted DNA possible going into downstream applications. Zymo Research has developed several lines of bisulfite conversion kits to enable DNA methylation analysis in challenging clinical samples and make bisulfite conversion fast and high throughput. The EZ DNA Methylation-Direct kits can perform DNA bisulfite conversion directly from blood, soft tissue, cells, FFPE, and LCM samples. These bisulfite conversion kits are compatible with low sample inputs and have been recently endorsed by many single cell sequencing protocols. EZ DNA Methylation-Lightning Kits include innovative technology and ready-to-use conversion reagents to streamline the bisulfite conversion process. These fast bisulfite conversion kits can finish converting samples in less than 1.5 hours with > 99.5% conversion efficiency. Additionally, low DNA input requirements and reduced fragmentation makes the EZ DNA Methylation-Lightning bisulfite conversion kits ideal for preparing targeted sequencing libraries for methylation analysis (validated by Roche NimbleGen SeqCap Epi), or whole genome bisulfite sequencing (WGBS) library preparation (Pico Methyl-Seq Library Prep Kit), as well as reduced representation bisulfite sequencing (RRBS) library preparation (Zymo-Seq RRBS Library kit). Designed to reduce template degradation, the EZ DNA Methylation bisulfite conversion kits minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. These bisulfite conversion kits are recommended with Illumina’s Infinium® Human Methylation 450K and MethylationEPIC Kits. Zymo Research has the best bisulfite conversion kits selection available, featuring streamlined workflows, ≥ 99% conversion rates, and low elution volumes for concentrated, bisulfite-converted DNA.