Bisulfite Conversion Kits

All bisulfite conversion kits are available in spin-column, 96-well, and MagBead formats. These innovative bisulfite conversion kits feature streamlined workflows, ≥ 99% conversion rates, automation friendly options, and low elution volumes for concentrated, bisulfite-converted DNA.

All MagBead kits are automation ready! Scripts available, contact

EZ DNA Methylation-Lightning
EZ DNA Methylation-Direct EZ DNA Methylation-Gold EZ DNA Methylation
Rapid workflow, most gentle chemistry Use directly with cells and tissues Classic bisulfite conversion Classic bisulfite conversion
Which kit is right for me? Best for new users and Illumina Array users Best for working with cells/tissue as direct input, and validated third party kits* Best for legacy users and users working with validated third-party kits** Best for legacy users and Illumina Array users
Conversion Efficiency > 99.5% > 99.5% > 99% > 99%
Input 100 pg – 2 µg of DNA DNA (≥ 50 pg), cells (≥ 10), blood, tissue, FFPE 500 pg – 2 µg of DNA 500 pg – 2 µg of DNA
Processing Time 1.5 hr 4 hr 3 hr 12-16 hr
Validated By Illumina MethylationEPIC Array (D5046, D5047, D5049) *IDT xGen Adaptase Module for Single-Cell Methyl-Seq **Agilent SureSelect Methyl-Seq, IDT xGen Methyl-Seq DNA Library Prep Kit Illumina MethylationEPIC Array (D5001, D5002, D5004)
Product Name
Overview - Bisulfite Conversion Kits

Sodium bisulfite conversion of DNA is the fastest, easiest, and most popular way to study DNA methylation analysis and selecting the right bisulfite conversion kit can be the difference between accurate data and frustrating wasted hours spent in the lab. Choosing a bisulfite conversion kit is an important step in DNA methylation studies to ensure that you obtain the highest quality bisulfite-converted DNA possible going into downstream applications. Zymo Research has developed several lines of bisulfite conversion kits to enable DNA methylation analysis in challenging clinical samples and make bisulfite conversion fast and high throughput. The EZ DNA Methylation-Direct kits can perform DNA bisulfite conversion directly from blood, soft tissue, cells, FFPE, and LCM samples. These bisulfite conversion kits are compatible with low sample inputs and have been recently endorsed by many single cell sequencing protocols. EZ DNA Methylation-Lightning Kits include innovative technology and ready-to-use conversion reagents to streamline the bisulfite conversion process. These fast bisulfite conversion kits can finish converting samples in less than 1.5 hours with > 99.5% conversion efficiency. Additionally, low DNA input requirements and reduced fragmentation makes the EZ DNA Methylation-Lightning bisulfite conversion kits ideal for preparing targeted sequencing libraries for methylation analysis (validated by Roche NimbleGen SeqCap Epi), or whole genome bisulfite sequencing (WGBS) library preparation (Pico Methyl-Seq Library Prep Kit), as well as reduced representation bisulfite sequencing (RRBS) library preparation (Zymo-Seq RRBS Library kit). Designed to reduce template degradation, the EZ DNA Methylation bisulfite conversion kits minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. These bisulfite conversion kits are recommended with Illumina’s Infinium® Human Methylation 450K and MethylationEPIC Kits. Zymo Research has the best bisulfite conversion kits selection available, featuring streamlined workflows, ≥ 99% conversion rates, and low elution volumes for concentrated, bisulfite-converted DNA.

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