The Quick-16S™ Full-Length Library Prep Kit (D6450) leverages optimized reagent chemistry and a streamlined workflow to offer users the accuracy and resolution of full-length 16S rRNA gene profiling in the same convenient and robust format of our short-read 16S library prep kits.

  • Speed - DNA to pooled libraries in 30 minutes of hands-on time.
  • Simplicity – One-step PCR with premixed amplification plate. Just add DNA.
  • Throughput – Multiplex up to 384 individual samples.
  • Flexibility – Compatible with both PacBio and Oxford Nanopore Technologies.

Streamlined Workflow

Total hands-on time for 96 samples: as little as 30 mins
Total hands-on time for 96 samples: as little as 30 mins

Reliable Read Distribution

Figure 1. Read Distribution Variation of Full-Length 16S Kit vs. Standard Protocol when processing fecal and soil sample DNA
Figure 1. Read Distribution Variation of Full-Length 16S Kit vs. Standard Protocol when processing fecal and soil sample DNA

Designed with Simplicity in Mind

Premixed reagents allow users to simply add DNA and amplify

Premixed reagents allow users to simply add DNA and amplify

qPCR compatibility enables easy QC and sample monitoring

qPCR compatibility enables easy QC and sample monitoring

Increased reagent stability allows for room temperature PCR setup

Increased reagent stability allows for room temperature PCR setup

Maximize Readcounts Using PacBio Kinnex™

Innovative primer design allows pooled libraries to be processed using either the PacBio Kinnex™ 16S rRNA Kit, or the standard SMRTbell® Prep Kit 3.0 when sequencing on Sequel II, IIe, & Revio™ platforms.

Figure 2. Kinnex™ 16S rRNA Kit vs. SMRTBell® Prep Kit 3.0 HiFi Reads. Concatenated amplicon sequencing via the Kinnex™ 16S rRNA kit demonstrates approximately 11-fold increase in HiFi reads generated, compared to single 16S gene sequencing.

Figure 2. Kinnex™ 16S rRNA Kit vs. SMRTBell® Prep Kit 3.0 HiFi Reads. Concatenated amplicon sequencing via the Kinnex™ 16S rRNA kit demonstrates approximately 11-fold increase in HiFi reads generated, compared to single 16S gene sequencing.

Accurate Microbial Profiling

Each Quick-16S™ Full-Length Library Prep Kit includes our ZymoBIOMICS® Microbial Community DNA Standard, which contains the extracted, quantified, and homogenized DNA of 8 organisms serving as a mock community.

Figure 3. Accurate profiling of ZymoBIOMICS® Microbial Community DNA Standard. Full-length 16S rRNA gene sequencing profiles accurately reflect the theoretical composition of the ZymoBIOMICS® Microbial Community DNA Standard, showing little-to-no amplification bias based on microbial genomic G/C content.

Figure 3. Accurate profiling of ZymoBIOMICS® Microbial Community DNA Standard. Full-length 16S rRNA gene sequencing profiles accurately reflect the theoretical composition of the ZymoBIOMICS® Microbial Community DNA Standard, showing little-to-no amplification bias based on microbial genomic G/C content.

Limited Time Offer

From now until November 15th, Zymo Research is offering beta testers of the Quick-16S™ Full-Length Library Prep Kit an opportunity to experience the advantages of full-length 16S rRNA gene sequencing, regardless of their access to a long-read platform. Beta test participants can choose to:

Receive a full-size kit to prepare and sequence their own libraries

OR

Receive an 8-prep kit and submit libraries for complementary PacBio sequencing

Don’t miss out on this exclusive offer and experience the power of full-length 16S sequencing for yourself!

Join the Beta Test
*Beta test is available to Domestic United States customers only, while supplies last.

16S rRNA gene sequencing is an essential technique for microbiome composition profiling, offering a cost-effective and robust alternative to shotgun metagenomics sequencing. This method requires less input DNA and is less impacted by non-microbial DNA presence. However, traditional short-read 16S sequencing suffers from reduced taxonomic resolution and identification biases due to partial gene sequencing.

Long-read sequencing platform developers such as PacBio® and Oxford Nanopore Technologies® (ONT) have enabled full-length 16S gene sequencing, which has revolutionized taxonomic profiling by making species or even strain-level identification possible. The Quick-16S™ Full-Length Library Prep Kit leverages a streamlined, robust workflow to deliver libraries that are optimized for SMRT™ sequencing on the Sequel II®, Sequel IIe®, & Revio® platforms. Prepped libraries boast additional compatibility with Kinnex library preparation for a dramatic increase in read depth on PacBio® platforms.

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