Scientific Posters
Zymo Research's scientific posters highlight the latest breakthroughs in molecular biology, showcasing innovations spanning from epigenetics to microbiomics. We are proud to present insights from our collaborations with leading scientists and institutions worldwide, with more contributions to the field of life science on the horizon.
Featured Poster
An Integrated Approach for Pathogen Detection, AMR Monitoring, and Functional Analysis in Wastewater
X. Cheng , J. Wilkinson, K. Ngo , P. Baybayan, Y. Kim, P. Pham, E. Carrasco, S. Tang, J. Shen, and K. LockenWastewater surveillance has emerged as a pivotal tool in public health epidemiology. Particularly catalyzed by the Covid-19 pandemic, modern culture-independent sequencing methods have become indispensable due to their ability to offer a comprehensive perspective.
View PosterSBP 39th Annual Symposium 2018
Yap Ching Chew, Wei Guo1, Xiaojing Yang, Paolo Piatti, Mingda Jin, Keith Booher, Michiko Suwoto, and Xi Yu JiaAging represents the most important risk factor for many chronic diseases including cardiovascular diseases, diabetes, and cancer, therefore understanding the mechanisms of aging is a fundamental...
View PosterSWARM Targeted Sequencing Measures Epigenetic Age
Wei Guo, Xiaojing Yang, Mingda Jin, Yap Ching Chew, Keith Booher, Steve Horvath, and Xi Yu JiaAging is the most important risk factor for many chronic diseases including cancer. DNA methylation is the most reliable molecular marker for aging quantification. However, genome-wide...
View PosterThe Epigenetic Clock Applies to Human Urine
Wei Guo, Steve Horvath, Michiko Suwoto, Yap Ching Chew, and Xi Yu JiaSeveral recent articles describe epigenetic biomarkers of aging based on methylation levels. For example, theepigenetic clock method (Horvath 2013, PMID: 24138928) is based on...
View PosterAn Integrated Approach for Pathogen Detection, AMR Monitoring, and Functional Analysis in Wastewater
X. Cheng, J. Wilkinson, K. Ngo, P. Baybayan, Y. Kim, P. Pham, E. Carrasco, S. Tang , J. Shen, and K. LockenWastewater surveillance has emerged as a pivotal tool in public health epidemiology. Particularly catalyzed by the Covid-19 pandemic, modern culture-independent sequencing methods have become indispensable due to their ability to offer a comprehensive perspective. In this study, we present an innovative, integrated approach for simultaneous pathogen detection, antimicrobial resistance (AMR) monitoring, and functional analysis within wastewater systems. By harnessing advanced sample preparation technologies provided by Zymo Research and leveraging PacBio Onso short-read sequencing, our objective is to elevate our comprehension of microbial dynamics within wastewater environments.
View PosterA Fast and Simple Method for Whole-Genome Bisulfite Sequencing Library Preparation from Ultra-low DNA input: Pico-MethylSeq Library Preparation Kit
Karolyn Giang, TzuHung Chung, Xueguang Sun, & Xi-Yu JiaThe distribution of 5-methylcytosine (5-mC) in DNA within the eukaryotic genome is known to greatly affect gene regulation and is currently a major topic of research. Studies on DNA methylation have been aided by advancements in bisulfite conversion and next-gen sequencing technologies which, when coupled, provide single-base resolution of 5-mC in the whole genome. Many whole-genome bisulfite sequencing (WGBS) library preparation protocols designed to analyze 5-mC distribution in the whole genome employ bisulfite to convert unmethylated cytosine bases to uracil after the library preparation and while these protocols produce reliable results, degradation of DNA is inherent to bisulfite conversion. As such, a large proportion of the adapterized library is fragmented and can no longer be amplified, which requires these protocols to call for large amounts of starting input DNA that is often times impossible to obtain. By rearranging the order of library preparation and bisulfite conversion, we developed a streamlined protocol that reveals whole-genome methylation patterns at single-base resolution. The work-flow leads with the degradation inherent to bisulfite conversion to randomly fragment the DNA prior to the library preparation, which allows the protocol to accommodate for pico-gram quantities of starting input, making it ideal for analysis in precious and limited samples. Comparisons of sequencing data from this WGBS library preparation method with the established Reduced Representation Bisulfite Sequencing method using human DNA showed a correlation coefficient of 0.95 for CpG sites with more than 10X coverage. With slight modifications, this protocol is versatile in its ability to prepare libraries for ChIP-seq and RNA-seq.
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