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    5-hmC Glucosyltransferase

    E2026 / E2027


    5-hmC Glucosyltransferase

    Cat # Name Size Price Quantity
    E2026 5-hmC Glucosyltransferase 100 U $125.00
    - +
    E2027 5-hmC Glucosyltransferase 200 U $207.00
    - +

    Documents


    Highlights
    • Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
    • Ideal for locus specific and global quantification of hydroxymethylated DNA.
    Description

    The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a
    glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.

    Concentration 2 U/µl
    Standard Reaction Time 2 hours
    Storage 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
    Unit Definition One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.