5-hmC Glucosyltransferase

E2026 / E2027


5-hmC Glucosyltransferase

E2026 / E2027

Cat # Name Size Price Quantity
E2026 5-hmC Glucosyltransferase 100 U $125.00
+ -
E2027 5-hmC Glucosyltransferase 200 U $207.00
+ -

Documents


5-hmC detection; 5-hmC enrichment

Highlights


  • Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
  • Ideal for locus specific and global quantification of hydroxymethylated DNA.

Description


The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.

Technical Specifications


Concentration 2 U/µl
Standard Reaction Time 2 hours
Storage 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
Unit Definition One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.