5-mC DNA ELISA Kit
D5325 / D5326
- For high-throughput detection of global 5-methylcytosine (5-mC) in DNA
- The streamlined workflow can be completed in less than 3 hours
The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.
|Detection||> 0.5% 5-mC per 100 ng DNA|
|Equipment||Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.|
|Input||This protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well.|
|Sample Source||Purified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.|
Q1: Why is there no color development after addition of HRP developer?
Q2: How to quantify the percentage of 5-mC in a DNA sample?
Q3: Can control DNAs be adjusted to low % 5-mC samples?
Q4: How should the components of the 5-mC ELISA kit be stored?
Q5: What is the shelf life of the ELISA kits?
Q6: Does the ELISA Kit detect methylated cytosine in non-CpG context?
Q7: Which wavelength should be used for the ELISA?
Q8: What is the minimum input amount of DNA per well?
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