a-Factor Mating Pheromone

Y1004-500


a-Factor Mating Pheromone

Y1004-500

Cat # Name Size Price Quantity
Y1004-500 a-Factor Mating Pheromone 500 µl $216.00
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Documents


Protocol: PDF   |   SDS (MSDS): PDF   |  
Aqueous solution for arresting yeast in G1 of the cell cycle

Highlights


  • Aqueous Solution: Convenient ready-to-use solution of yeast a-factor mating pheromone for mating induction and G1 cell cycle arrest.
  • Robust and Efficient: Liquid solution has been optimized for both activity and stability and is guaranteed to retain biological function through multiple freeze-thaw cycles.
  • Easy: Widely-used simple method for studying the cell cycle, cellular morphology, transcriptional induction, and signal transduction pathways.

Description


When yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes, affectionately termed "schmooing". These alterations prepare the yeast cells for mating and fusion to form stable diploids. The a/alpha diploids are not responsive to mating pheromone of either type, but can be induced to undergo meiosis via nutrient deprivation. The use of yeast mating pheromones has pioneered the study of the cell cycle, cellular morphology, transcriptional induction, as well as signal transduction pathways. Zymo Research provides the a-factor peptide mating pheromone as a ready to use liquid that has been optimized for both activity and stability and is guaranteed to retain biological function through multiple freeze-thaw cycles.

Technical Specifications


Concentration 1 mg/ml in methanol
Molecular Weight 1630.0 Da
Product Storage Store at -20°C. a-factor is stable during multiple freeze-thaw cycles.
Purity > 80% by HPLC
Structure a-Factor is a farnesylated dodecapeptide
Usage We recommend using the a-factor at concentrations of 0.5 µg/ml (BAR1Δ). However, BAR1 strains have not been tested. To dilute the a-factor to working concentrations, we recommend using 0.5 mg/ml BSA (bovine serum albumin) in water.

Citations


Alpha-factor mating pheromone was used to arrest and synchronize yeast cells at the G1 phase while expression of CopN protein was induced. Synchronization resulted in a homogenous cell culture which was important for studying the effects CopN had on cell cycle progression.

Huang, J., et al. (2008). The essential role of the CopN protein in Chlamydia pneumonia intracellular growth. Nature, 456: 112-115