Atlantis dsDNase

E2030


Atlantis dsDNase

E2030

Cat # Name Size Price Quantity
E2030 Atlantis dsDNase 12.5U $48.00
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Documents


Digestion of dsDNA

Highlights


  • Ideal for use in nucleosome mapping studies.
  • Sold separately or as part of EZ Nucleosomal DNA Prep Kit (D5220).

Description


Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.

Technical Specifications


Concentration 0.1 U/µl
Inactivation 5X MN Stop Buffer or EDTA
Standard Reaction Time 20 minutes
Storage Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤-70°C.
Unit Definition One unit (U) is defined as the amount of enzyme needed to produce an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).

Product FAQ


Q1: What is the sensitivity of the enzyme to different cell types?

Q2: What is the difference between Atlantis dsDNase and MNase?

Q3: Do the sticky-end fragments have 5’ vs. 3’-overhangs? What is the relative abundance of different ends?

Q4: What if the nucleosomal DNA is not shearing properly and the majority of DNA is still intact?