- Ideal for use in nucleosome mapping studies.
- Sold separately or as part of EZ Nucleosomal DNA Prep Kit (D5220).
Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
|Inactivation||5X MN Stop Buffer or EDTA|
|Standard Reaction Time||20 minutes|
|Storage||Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤-70°C.|
|Unit Definition||One unit (U) is defined as the amount of enzyme needed to produce an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).|
Q1: What is the sensitivity of the enzyme to different cell types?
Q2: What is the difference between Atlantis dsDNase and MNase?
Q3: Do the sticky-end fragments have 5’ vs. 3’-overhangs? What is the relative abundance of different ends?
Q4: What if the nucleosomal DNA is not shearing properly and the majority of DNA is still intact?
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