DNA Clean & Concentrator-100
D4029 / D4030
- Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
- Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
|Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
|Elution Volume||≥ 150 µl|
|Equipment||Microcentrifuge and centrifuge or vacuum source.|
|Purity||Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.|
|Sample Source||DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.|
|Size Range||50 bp to 23 kb|
|Yield||≤ 100 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Q2: How to process naked DNA stored in DNA/RNA Shield?
Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How many times can columns be reloaded?
Q6: What is the minimum input volume of DNA sample?
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”
- Kathleen B. (SUNY-ESF)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
- Tyler C.
“It was a very simple procedure and it gave a concentration ten times the original amount.”
- Kimberly M. (University of Pennsylvania)
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