DNA Clean & Concentrator-25
D4033 / D4034 / D4005 / D4006
- Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
- Column design allows DNA to be eluted at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
|Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
|Elution Volume||≥ 25 µl|
|Purity||Highly-pure DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.|
|Sample Source||DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.|
|Size Range||50 bp to 23 kb|
|Yield||≤ 25 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
Q1: What is the minimum input volume of DNA sample?
Q2: How many times can columns be reloaded?
Q3: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q4: How can I process naked DNA stored in DNA/RNA Shield?
Q5: What is the lower limit and minimal amount of DNA that can be recovered?
Q6: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA Clean & Concentrator-25 was used to purify cDNA library for Illumina small RNA deep sequencing in the analysis of miRNA transfected cells. Results showed varying concentrations of miRNA mimics caused non-specific alterations in gene expression and that guide strands were frequently mutated.Jin HY, et al. (2015). Transfection of microRNA Mimics Should Be Used With Caution. Front. Genet. 6:340.
PCR products were purified using the DNA Clean & Concentrator-25, followed by Sanger multilocus sequence analysis and confirmed by repetitive PCR; this revealed the high diversity of C. michiganensis subspecies, an agent of bacterial canker of tomato.Tancos MA, et. al. (2015). Characterizing the Genetic Diversity of the Clavibacter michiganensis subsp. Michiganensis Population in New York. Phytopathology. 105(2): 169-179.
The DNA Clean & Concentrator-25 was used to purify genomic DNA from different phytoplankton species extracted using a CTAB/chloroform method. Researchers found that bead-beating improved DNA yield by as much as 2 fold and that DNA integrity was preserved by subsequent qPCR analysis and cloning of the ITS region.Yuan J, et. al. (2015). An Improved DNA Extraction Method for Efficient and Quantitative Recovery of Phytoplankton Diversity in Natural Assemblages. PLoS ONE. 10(7).
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”
- Kathleen B. (SUNY-ESF)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
- Tyler C.
“It only took 2 minutes for the total procedure - shorter than my current method of PCR purification.”
- Marissa V. (Harvard Medical School)