DNA Degradase Plus

E2020 / E2021


DNA Degradase Plus

E2020 / E2021

Cat # Name Size Price Quantity
E2020 DNA Degradase Plus 250 U $136.00
+ -
E2021 DNA Degradase Plus 1000 U $435.00
+ -

Documents


Complete digestion of DNA into individual nucleosides.

Highlights


  • Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
  • Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol

Description


DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

Technical Specifications


Assay Condition DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour
Concentration 5 U/µl
Enzyme Inactivation Heat inactivate enzyme at 70C for 20 minutes
Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition 5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr

Product FAQ


Q1: Can I visualize the treated samples/ do I need to purify after Degradase treatment?

Q2: Can I let the reaction go longer than stated in the protocol?

Q3: Can I add excess enzyme to the reaction?

Q4: Does this work with RNA/ ssDNA?

Q5: Can I scale up/ down the reaction volumes? How do I do that?

Citations