DNA Degradase Plus
E2020 / E2021
- Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
- Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
|Assay Condition||DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour|
|Enzyme Inactivation||Heat inactivate enzyme at 70C for 20 minutes|
|Storage||Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.|
|Unit Definition||5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr|
Q1: Can I visualize the treated samples/ do I need to purify after Degradase treatment?
Q2: Can I let the reaction go longer than stated in the protocol?
Q3: Can I add excess enzyme to the reaction?
Q4: Does this work with RNA/ ssDNA?
Q5: Can I scale up/ down the reaction volumes? How do I do that?
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