|Applicable For||Next-Generation Sequencing, qPCR, microarray|
|Device Specs||12 x 80 mm screwcap vial pre-filled with DNA/RNA Shield (1 mL or 2 mL)|
|Device Storage||Ambient temperature => 2 years|
|Sample Collection||10% (v/v or w/v)|
|Sample Source||Nose, mouth, throat, biological fluids, environmental samples|
|Sample Stability||RNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (<-20°C): Indefinitely
Q1: How to collect solid tissues in DNA/RNA Shield?
Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Q3: What sample types is DNA/RNA Shield suitable for?
Q4: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
Q5: How long can samples be stored frozen in DNA/RNA Shield?
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
Q8: Can I FACS sort directly into DNA/RNA Shield?
During a survey of Middle East respiratory syndrome coronavirus (MERS-CoV), nasal swabs from dromedary camels were collected into DNA/RNA Shield . Any MERS-positive swab samples were completely inactivated in DNA/RNA Shield and were subsequently used for RNA extraction. Successful detection of MERS-CoV and phylogenetic analysis suggests local zoonotic transmission through the respiratory route onto humans.Nowotny N, et. al. (2014). Middle east Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels, Oman, 2013. Eurosurveillance. 19(16).
Human nasopharyngeal swab samples were stored in DNA/RNA Shield and nucleic acids were extracted using the EasyMAG NucliSENS automated system from bioMerieux for downstream RT-PCR. Testing against a respiratory viral panel showed that 7.2% of samples tested positive consisting of rhinovirus and coronavirus.Shaman, J et al. Asymptomatic Summertime Shedding of Respiratory Viruses. Journal of Infectious Diseases. 2018.
Surfaces of a kimchi production environment were swabbed and samples were stored in DNA/RNA Shield Lysis Tubes for later extraction using the ZymoBIOMICS DNA kit. Targeted 16S rRNA gene sequencing of the V4 hypervariable region showed that the bacterial communities are similar between vegan and non-vegan products using fermented seafood ingredients.Zabat, MA et al. The impact of vegan production on the kimchi microbiome. Food Microbiology. 2018.
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)