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DNA/RNA Shield DirectDetect
R1400 / R1401-1 / R1400-E / R1401-1-E
DNA/RNA Shield DirectDetect
- Eliminates the need for RNA (or DNA) extraction, allowing for rapid, direct, cost-effective analysis of samples
- No inhibition of real-time PCR
- Reduces sample viscosity to minimize pipetting errors with automated liquid handlers
DNA/RNA Shield DirectDetect is a reagent that allows users to go from sample collection directly to PCR. DNA/RNA Shield DirectDetect safeguards viral genomes (nucleic acids) from degradation and provides ambient temperature collection and transportation of samples without the need for nucleic acid extraction.*
*DNA/RNA Shield DirectDetect does not inactivate pathogens. For inactivating transport media, see DNA/RNA Shield.
|Applicable For||RT-PCR, COVID-19 Testing|
|Reagent Storage||Ambient temperature => 1 year|
|Sample Collection||Swab (1 swab/mL), Saliva (50% v/v)|
|Sample Source||Saliva, oral (and nasal) specimen|
|Sample Stability||Ambient Temperature (4°C-25°C)
< 1 week
Q1: Can samples stored in DNA/RNA Shield be combined with DNA/RNA DirectDetect for bypassing sample extraction?
No, these are two separate reagents. Once a sample is collected in DNA/RNA Shield it cannot bypass extraction, it must be collected in DNA/RNA Shield DirectDetect in order to work as advertised.
Q2: Is DNA/RNA Shield DirectDetect the same as DNA/RNA Shield?
No, DNA/RNA Shield DirectDetect does not inactivate pathogens while DNA/RNA Shield does. Additionally, DNA/RNA Shield DirectDetect preserves nucleic acid for one week, while DNA/RNA Shield preserves RNA for at least 30 days and DNA for at least 2 years.
In India, where testing supplies and vaccinations are difficult to obtain, testing is imperative to diagnosing the SARS-CoV-2 virus. While many biotechnology and testing suppliers have tended to concentrate on their domestic testing efforts, we appreciate Zymo Research's global humanitarian effort to help eradicate COVID-19.
- Anu Acharya, Founder and CEO of Mapmygenome
DNA/RNA Shield - DirectDetect enables countries seeing an increase in COVID-19 cases to perform COVID-19 testing without extraction; therefore, removing the barrier of limited extraction resources. It's our way of providing humanitarian support to people in need. We teamed up with Mapmygenome because of their ability to rapidly deploy and analyze RT-PCR testing.