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    DNase I Set

    E1010 / E1011 / E1012

    Get Free Sample Limited time offer!

    DNase I Set

    Get Free Sample Limited time offer!
    Cat # Name Size Price Quantity
    E1010 DNase I Set 250 U $63.40
    - +
    E1011 DNase I Set 1500 U $132.90
    - +
    E1012 DNase I Set 5 x 1500 U $596.40
    - +

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    • Superior activity.
    • Certified RNase-free and protease-free.
    • Withstand more than 10 freeze-thaw cycles.

    DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA. It requires divalent metal cations to be active.

    1Unit Definition One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
    2Storage After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
    3Reconstitution Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
    4Inactivation Heat inactivate at 75 °C for 10 min with 5 mM EDTA.

    • Remove DNA from protein and RNA samples.
    • Preparation of DNA-free RNA.
    • DNA labeling by nick-translation.
    • Removal of DNA template after in vitro transcription.
    • Removal of DNA from RNA samples prior to downstream applications (NextGen sequencing, RT-PCR).

    Cat # Name Size Price
    E1010-1-4 DNA Digestion Buffer 4 mL $17.00
    E1010-1-16 DNA Digestion Buffer 16 mL $32.90