Successfully Added to Cart

Updated Cart Total: 0 items Subtotal: $0.00 USD
View Cart

Customers also bought...

    Complimentary shipping on orders over $750

    dsDNA Shearase Plus (Includes Matching DCC-5 Kit)

    Highlights

    • The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
    • Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
    • Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Highlights

    • The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
    • Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
    • Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size Price Quantity
    E2019-200 dsDNA Shearase Plus (Includes Matching DCC-5 Kit) 200 U $722.40
    - +
    E2019-50 dsDNA Shearase Plus (Includes Matching DCC-5 Kit) 50 U $199.40
    - +
    Cat # Name Size Price
    E2018-50 dsDNA Shearase Plus 50 U $123.10
    D4013 DNA Clean & Concentrator-5 (Capped) 50 Preps $94.60

    Description

    dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.

    Performance

    Technical Specifications
    Concentration 1 U/µl
    Enzyme Inactivation Heat in activate enzyme at 65C for 5 min.
    Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
    Unit Definition One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42C in total reaction volume in 10 µl.

    Resources

    Documents

    Citations

    Need help? Contact Us