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Format
Femto Fungal DNA Quantification Kit
Highlights
- Quantify as little 20 femtograms of fungal DNA in as little as 1 µl of sample.
- High specificity and sensitivity for fungal DNA in a background of non-target DNA.
- Fast and simple: add samples to the PreMix... and quantify.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Format
Femto Fungal DNA Quantification Kit
Highlights
- Quantify as little 20 femtograms of fungal DNA in as little as 1 µl of sample.
- High specificity and sensitivity for fungal DNA in a background of non-target DNA.
- Fast and simple: add samples to the PreMix... and quantify.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Type | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E2007-1 | Femto Fungal qPCR Premix | Bacterial DNA | $218.30 | |
Description
Performance
Technical Specifications
| Detection Range | 20 fg to 20 ng DNA |
|---|---|
| Equipment | Real-time quantitative PCR system, vortex mixer, microcentrifuge, PCR tube strip or PCR plate; optically transparent sealing film for PCR plate or tube strip caps |
| Sample Source | Purified DNA |
Resources
Documents
FAQ
Since this kit is so sensitive, avoiding possible sources of contamination is key for a successful qPCR. - Work in a clean environment and decontaminate the benchtop and pipettes with a 10% bleach solution. - Wipe down the areas with ethanol to remove the trace amounts of bleach and allow the area to try. - Use filtered tips. - Take care to ensure that pipetting is efficient and accurate. Calibrate your pipettes regularly. - Add standards directly into the premix and pipet up and down to flush out any remaining liquid from the tip. - Avoid pipetting onto the walls as this may skew the results. - The SYTO9 dye present within the premix is sensitive to light, so working quickly and diligently is recommended.
Our qPCR Premixes does not contain ROX Reference Dye. Ensure that the ROX channel of your device is switched off.
Our qPCR Premixes contains Syto9. You can use the SYBR Green channel for signal detection.
No, kits are not certified.
Primers are ITS1-F and ITS2-R. Forward Primer: ITS-1F 5'- CTTGGTCATTTAGAGGAAGTAA-3' Reverse Primer: ITS-2R 5'- GCTGCGTTCTTCATCGATGC-3' They are based on this publication: White, Thomas J., et al. "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics." PCR protocols: a guide to methods and applications 18 (1990): 315-322
The kit targets the ITS region
The DNA is derived from S. cerevisiae strain TMY18.
Citations
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