- Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
- Unique spin column for low volume elution of ultra-pure, high-yield DNA.
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
|Applicable For||Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application|
|Elution Volume||≥ 10 µl of DNA Elution Buffer|
|Purity||A260/A280 > 1.8, A260/A230 > 1.8|
|Sample Source||Enzymatic reactions or impure preparations containing genomic DNA.|
|Sample Storage||Eluted DNA can be used immediately or stored at ≤ -20°C.|
|Size Range||50 bp to 200 kb|
|Yield||Recovery of DNA ranges from 70 - 95%|
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Q2: How can I process naked DNA stored in DNA/RNA Shield?
Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How many times can columns be reloaded?
Q6: What is the minimum input volume of DNA sample?
The Genomic DNA Clean & Concentrator-10 was used to clean gDNA extracted from human whole blood samples. Results showed high prevalence of asymptomatic infections and misdiagnosis of falciparum and vivax malaria in Tak parasite. This indicates higher than estimated transmission levels and the need for more sensitive malaria detection assays.Baum E, et al. (2015). Submicroscopic and asymptomatic Plasmodium falciparum and Plasmodium vivax infections are common in western Thailand – molecular and serological evidence. Malaria Journal. 14(1): 95.
The Genomic DNA Clean & Concentrator was implemented for the purification of whole genome amplified DNA prior to generating DNA standards that are differentially methylated. This allowed accurate quantitation of methylation levels at the estrogen receptor alpha (ESR1) gene locus in the bovine endometrium.Furst RW, et al. (2012) Is DNA methylation an epigenetic contribution to transcriptional regulation of the bovine endometrium during the estrous cycle and early pregnancy? Mol Cell Endocrinol. 348(1): 67-77