Genomic DNA Clean & Concentrator-10

D4011 / D4010


Genomic DNA Clean & Concentrator-10

D4011 / D4010

Cat # Name Size Price Quantity
D4011 Genomic DNA Clean & Concentrator-10 100 Preps $298.00
+ -
D4010 Genomic DNA Clean & Concentrator-10 25 Preps $91.00
+ -

Documents


Purify high molecular weight DNA from enzymatic reactions and dilute samples.

Highlights


  • Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
  • Unique spin column for low volume elution of ultra-pure, high-yield DNA.
  • Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.

Description


The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.

Technical Specifications


Applicable For Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume ≥ 10 µl of DNA Elution Buffer
Equipment Microcentrifuge
Purity A260/A280 > 1.8, A260/A230 > 1.8
Sample Source Enzymatic reactions or impure preparations containing genomic DNA.
Sample Storage Eluted DNA can be used immediately or stored at ≤ -20°C.
Size Range 50 bp to 200 kb
Yield Recovery of DNA ranges from 70 - 95%

Product FAQ


Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Q2: How can I process naked DNA stored in DNA/RNA Shield?

Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Q5: How many times can columns be reloaded?

Q6: What is the minimum input volume of DNA sample?

Citations


The Genomic DNA Clean & Concentrator-10 was used to clean gDNA extracted from human whole blood samples. Results showed high prevalence of asymptomatic infections and misdiagnosis of falciparum and vivax malaria in Tak parasite. This indicates higher than estimated transmission levels and the need for more sensitive malaria detection assays.

Baum E, et al. (2015). Submicroscopic and asymptomatic Plasmodium falciparum and Plasmodium vivax infections are common in western Thailand – molecular and serological evidence. Malaria Journal. 14(1): 95.

The Genomic DNA Clean & Concentrator was implemented for the purification of whole genome amplified DNA prior to generating DNA standards that are differentially methylated. This allowed accurate quantitation of methylation levels at the estrogen receptor alpha (ESR1) gene locus in the bovine endometrium.

Furst RW, et al. (2012) Is DNA methylation an epigenetic contribution to transcriptional regulation of the bovine endometrium during the estrous cycle and early pregnancy? Mol Cell Endocrinol. 348(1): 67-77

Kit Components