Genomic DNA Clean & Concentrator-25
D4064 / D4065
- Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
- Unique spin column for low volume elution of ultra-pure, high-yield DNA
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
The Genomic DNA Clean & Concentrator-25 (DCC) is a spin column gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
|Applicable For||Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application|
|Elution Volume||≥ 35 µl of DNA Elution Buffer|
|Purity||A260/A280 > 1.8, A260/A230 > 1.8|
|Sample Source||Enzymatic reactions or impure preparations containing genomic DNAs|
|Sample storage||Eluted DNA can be used immediately or stored at ≤ -20°C|
|Size Range||50 bp to 200 kb|
|Yield||Recovery of DNA ranges from 70 - 95%|
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Q2: How to process naked DNA stored in DNA/RNA Shield?
Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How many times can columns be reloaded?
Q6: What is the minimum input volume of DNA sample?
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