Genomic DNA Clean & Concentrator-25

D4064 / D4065


Genomic DNA Clean & Concentrator-25

D4064 / D4065

Cat # Name Size Price Quantity
D4064 Genomic DNA Clean & Concentrator-25 25 Preps $91.00
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D4065 Genomic DNA Clean & Concentrator-25 100 Preps $298.00
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Genomic DNA Clean & Concentrator-25 (DCC) 25

Highlights

  • Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
  • Unique spin column for low volume elution of ultra-pure, high-yield DNA
  • Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Description

The Genomic DNA Clean & Concentrator-25 (DCC) is a spin column gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.


Applicable For Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume ≥ 35 µl of DNA Elution Buffer
Equipment Microcentrifuge
Purity A260/A280 > 1.8, A260/A230 > 1.8
Sample Source Enzymatic reactions or impure preparations containing genomic DNAs
Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
Size Range 50 bp to 200 kb
Yield Recovery of DNA ranges from 70 - 95%

Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q2: How to process naked DNA stored in DNA/RNA Shield?

Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).

Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?

The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How many times can columns be reloaded?

We recommend no more than 5 times as binding efficiency might decrease.

Q6: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.



Cat # Name Size Price
D3004-4-1 DNA Elution Buffer 1 ml $11.00
D3004-4-10 DNA Elution Buffer 10 ml $14.00
D3004-4-4 DNA Elution Buffer 4 ml $10.00
D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml $33.00
D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml $10.00
D5201-1-50 ChIP DNA Binding Buffer 50 ml $37.00
C1001-50 Collection Tubes 50 Pack $15.00
C1001-500 Collection Tubes 500 Pack $52.00
C1001-1000 Collection Tubes 1000 Pack $90.00
C1102-25 Zymo-Spin IIC-XL Columns 25 Pack $34.00