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Read The Zymo Research PromiseLoad N’ Go Quick-DNA/RNA HT Kit
Cat # | Name | Size | Price | Quantity |
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Highlights
- Save Time and Focus on Discovery: Pre-filled 96-well reagent plate technology that offers multi-platform compatibility and reduces hands-on time by 75%.
- Versatile Nucleic Acid Extraction for Various Samples: Purify high-quality DNA easily and reliably from any biological fluid, cultured/monolayer cells, or tissue sample.
- Precision Meets Versatility: The Zymo magbead purification system ensures DNA and RNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
Documents
Product Description
Technical Specifications
Binding Capacity | 5 µg DNA/RNA per 10 µl MagBinding Beads |
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Elution Volume | ≥ 15 µl DNase/RNase-Free Water |
Purity | DNA/RNA is ready for Next-Gen Sequencing, RT/qPCR, etc. |
Sample Sources | Any biological sample (e.g., swabs, liquids, cells, tissue, etc.), and samples collected/stored in media (e.g., UTM, VTM, saline, PBS, DNA/RNA Shield, PAXgene, RNAlater, RNAProtect, etc.) |
Storage Temperature and Stability |
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Resources
Q1: Can the Load N' Go Quick-DNA/RNA HT kit be used with different laboratory automation platforms, and is there a specific protocol or script needed for compatibility?
Yes, the Load N' Go Quick-DNA/RNA HT kit is compatible with various laboratory automation platforms. Its design facilitates seamless integration into high-throughput workflows, catering to both manual and automated processing. While the kit is generally compatible with most platforms, it is recommended to consult with the automation team from Zymo Research for appropriate scripts and scripting support. You can contact them at automation@zymoresearch.com to ensure optimal performance and compatibility with your chosen laboratory automation system.
Q2: Can I still use DNA/RNA Shield if a precipitate has formed?
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
Q3: What are the main applications for this kit?
Virus detection is one of the dominant applications for this kit. Other dominant applications include infectious disease, microbiomics, cancer research, and more.
Q4: Is it possible to use this kit with veterinary samples?
Yes, there are no restrictions to this kit on veterinary samples.
Q5: Does this kit extract DNA and RNA in two separate fractions?
No. This kit extracts total nucleic acids (DNA & RNA) together in the same fraction.
Q6: What downstream applications can be used with DNA and RNA in the same fraction?
Total nucleic acid (DNA/RNA) extracted can be directly used for any DNA application. For RNA applications, include DNase I treatment if it is necessary to the downstream analysis.
Q1: Should RNAlater be removed before processing?
Yes, it is recommended to remove RNAlater prior to processing. However, when removal is not possible, the input should be diluted. For example, use 50 µl of input per prep and add 750 µl of DNA/RNA Buffer HT at step 1 under (III) DNA/RNA Purification on page 7 in the instruction manual.
Q2: If the input is in TRIzol or Tri-Reagent, how should it be processed?
If the input is in TRIzol or TRI-Reagent, it is recommended to add chloroform and process the sample to produce the aqueous phase. Carefully, transfer the aqueous phase into a new tube and process with the kit (see sample preparation). To process input in TRIzol or TRI-Reagent directly, it is recommended to use the Direct-zol-96 MagBead RNA kit (R2100, R2101, R2102, R2103).
Q3: How would this kit work for detecting bacterial samples?
For unbiased bacterial detection, proper homogenization with the ZR BashingBead Lysis Tubes (S6012-50) is recommended before continuing to nucleic acid extraction with the kit.
Q1: How can we use this kit with automated, robotic type platforms (i.e., Tecan, KingFisher, Hamilton, etc.)?
This kit can be directly used with all robotic-type sample processors (i.e., bead movers) for nucleic acid extraction from clinical samples. Detailed specifications and steps can vary based on sample type and automation platform used. For automation scripts and related technical support, please email automation@zymoresearch.com for free consultation.
Q2: Do you have a list of compatible automated machines?
Compatible automated machines include, but are not limited to:
- Allsheng Autopure
- Accuris Isopure 96
- Kingfisher Flex
- Kingfisher Apex
- Tecan Fluent
- Hamilton Microlab Star
- Opentrons OT-2
Additionally, the kit is also compatible with 8-16 prep platforms, such as:
- KingFisher Duo/Duo Prime
- IsoPure Mini
Please note that only the standard format (R2150, R2150-E, R2151, R2151-E) of this kit is compatible with these smaller platforms, as the Load N’ Go format (R2152) is only compatible with 96-prep format platforms.
Q3: For automated platforms not listed, can Zymo Research validate my specific automated platform?
We can conduct proof-of-principle testing to validate the kit’s chemistry on any platform we have in-house. If we do not have the preferred platform at our disposal, we can send a free kit for the purpose of workflow validation and provide support with scripting and troubleshooting as needed at no cost.
Q4: Is there any information comparing the speed of the Load N’ Go Quick-DNA/RNA HT Kit (R2152) compared to other competitor kits on each open platform?
No other kit currently on the market can match the capability of this assay in a pre-filled format. Generally, it takes 45-50 minutes to perform the assay, depending on the platform.
Q5: Do you have yield and time comparison between the Load N’ Go Quick-DNA/RNA HT Kit (R2152) and the Mag-Bind® Viral DNA/RNA Xpress Kit CE IVD (Omega Bio-tek)?
Currently, no. The Omega Bio-tek kit on the market does not match the capability of this assay in a pre-filled format to make a direct comparison. From our testings, there is no significant difference in yield between the Load N’ Go format or our standard format for this assay. Yield will vary depending on sample type and condition of the specific sample.
Q6: For the Load N’ Go Quick-DNA/RNA HT Kit (R2152), will the scripts contain information on where to place each plate on platform?
Yes. The delivery of the layout information will vary depending on the platform, and usually the user will be prompted by their platform to place the plates in a specific order/layout. If a platform does not directly prompt the user, we will provide supplemental materials detailing the layout.
Cat # | Name | Size | Price | |
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R2150-1-100 | DNA/RNA Buffer HT | 100 mL | $179.30 | |
D3001-2-60 | Proteinase K w/ Storage Buffer Set | 60 mg | $149.60 | |
C2019 | 96 Tip Combs (For V-Bottom Deep Well Plate) | 2 Combs | $14.00 | |
R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | $248.20 | |
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