Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
|Enzyme Commission Number||(E.C. 184.108.40.206)|
|Enzyme Inactivation||EDTA or EGTA in molar excess of CaCl2.|
|Storage||Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.|
|Unit Definition||One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.|