Micrococcal Nuclease

D5220-1


Micrococcal Nuclease

D5220-1

Cat # Name Size Price Quantity
D5220-1 Micrococcal Nuclease 10 U/100 µl $24.00
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Micrococcal Nuclease

Description


Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.

Technical Specifications


Concentration 0.1 U/µl
Enzyme Commission Number (E.C. 3.1.31.1)
Enzyme Inactivation EDTA or EGTA in molar excess of CaCl2.
Storage Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.
Unit Definition One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.

Product FAQ


Q1: What is the difference between Atlantis dsDNase and MNase?

Q2: What is the sensitivity of the enzyme to different cell types?

Q3: Is there information on whether the exonuclease activity of the MNase digest dsDNA from 3' to 5'?