Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
|Enzyme Commission Number||(E.C. 220.127.116.11)|
|Enzyme Inactivation||EDTA or EGTA in molar excess of CaCl2.|
|Storage||Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.|
|Unit Definition||One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.|
Q1: What is the difference between Atlantis dsDNase and MNase?
Q2: What is the sensitivity of the enzyme to different cell types?
Q3: Is there information on whether the exonuclease activity of the MNase digest dsDNA from 3' to 5'?
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