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    OneStep PCR Inhibitor Removal Sample Kit (5 preps)


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    Highlights

    • Simplified Workflow
    • Improved stability and efficiency
    • Removes polyphenolics that cause PCR inhibition such as Humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
    • Fast, one-step procedure for cleaning impure samples prior to PCR, Sequencing, Reverse transcription (RT), etc.
    Description & Documents Specifications FAQ

    Documents

    Product Description

    The OneStep PCR Inhibitor Removal kits are PCR inhibitor clean up kits that contain all the components needed for efficient removal of contaminants from DNA and RNA preparations that can inhibit downstream enzymatic reactions (e.g. PCR and RT). The column slurry has been efficiently designed to bind and remove any polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean up is as simple as filtering your sample with prepped column.

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    Technical Specifications

    Applicable for Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc
    Input Volume 50-200µl
    Equipment Microcentrifuge
    Purity High quality, enzymatic-reaction ready DNA/RNA products.
    Sample source Samples contaminated by polyphenolics that inhibit enzymatic reactions.
    Sample storage Eluted DNA/RNA can be used immediately or stored at ≤ - 20°C.
    Type ds/ssDNA and RNA.
    Yield 80-90%

    Resources

    The minimum volume that can be processed with the HRC columns is 50µl. For the maximum volume, the columns can handle as much as 200µl, however, the input limit is not as dependent on the column as it is on the inhibitor presence in the sample. For largely inhibited samples it is recommended to keep volumes between 50-100µl.

    The new version has a more simplified workflow as it does not require any prep buffer and has improved stability for more efficient inhibitor removal.

    There may be residual buffer from the column in your sample, however, all the components in the column are inert and will not affect any downstream applications.

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