Quest 5-hmC DNA ELISA Kit

D5425 / D5426


Quest 5-hmC DNA ELISA Kit

D5425 / D5426

Cat # Name Size Price Quantity
D5425 Quest 5-hmC DNA ELISA Kit 1 X 96 Preps $423.00
+ -
D5426 Quest 5-hmC DNA ELISA Kit 2 X 96 Preps $672.00
+ -

Documents


Quest 5-hmC DNA ELISA Kit

Highlights


  • Sensitive and specific detection of 5-hydroxymethlycytosine (5-hmC) from a variety of samples
  • Ideal for global 5-hmC quantitation and high throughput screening
  • Streamlined workflow can be completed in 3 hrs

Description


The Quest 5-hmC DNA ELISA Kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as well as enzyme-digested and mechanically sheared fragments. The Control DNA Set included with this kit has been calibrated to accurately quantify the percent 5-hmC in sample DNA by use of a standard curve. Also, the fast, streamlined workflow is ideal when analyzing/screening large numbers of samples.

Technical Specifications


Detection ≥ 0.02% 5-hmC per 100 ng DNA
Equipment Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.
Input This protocol is optimized for 100 ng of input DNA/well. Compatible with DNA in the range of 20-200 ng/well.
Sample Source Purified genomic DNA in water, Tris-EDTA, or similar.

Product FAQ


Q1: What is the shelf life of the ELISA kits?

Q2: How to store the components of the 5h-mC ELISA kit?

Q3: Where are the Control DNA derived from?

Q4: Which wavelength should be used for the ELISA?

Q5: How to quantify the percentage of 5-hmC in a DNA sample?

Q6: Why is there no color development after addition of HRP developer?

Q7: My ODs so low. What should I check?

Citations


Kit Components