The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
100% automation ready with only a single PCR step and without the need for normalization.
Real-time PCR enables absolute microbial copy number quantification.
Description & Documents
Specifications
Components
Documents
Product Description
The Quick-16S Plus NGS Library Prep Kit (V1-V3) is the fastest and simplest NGS library prep targeting the V1-V3 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
16S V1-V3 Primer Sequences (adapters not included)
27f (AGRGTTYGATYMTGGCTCAG, 20 bp) and 515r (TBACCGCGGCTGCTGGCAC, 19 bp).
Amplicon Size
The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~492 bp.
Barcode Sequences
10 bp barcodes are available to download here or by visiting the Documentation section of this Product Page.
Index Primers
Unique dual index (barcodes) to label individual samples.
Required Equipment
Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample Input
Purified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing Platform
Illumina MiSeq® or NextSeq 1000/2000® without the need to add custom sequencing primers. We recommend the MiSeq® Reagent Kit v3 (600-cycle) or NextSeq 1000/2000® Reagent Kit P1/P2 (600-cycle). For assistance with sample sheet setup, see Appendix F.
DNA/RNA Shield (50 ml)Cat#: R1100-50DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the...
DNA/RNA Shield SafeCollect Swab Collection Kit, 1ml (1 collection kit)Cat#: R1160The DNA/RNA Shield SafeCollect Swab Collection Kit is a user-friendly collection kit for stabilizing the nucleic acid content of samples collected with a swab. DNA/RNA Shield completely inactivates harmful pathogens...
