Quick-cfRNA Serum & Plasma Kit

R1059


Quick-cfRNA Serum & Plasma Kit

R1059

Cat # Name Size Price Quantity
R1059 Quick-cfRNA Serum & Plasma Kit 50 preps $355.00
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Documents


Protocol: PDF   |   SDS (MSDS): PDF   |   White paper: PDF   |  
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Highlights


  • Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
  • Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
  • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.

Description


The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.

Technical Specifications


Elution Volume cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Type Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size RNA ≥ 17 nt.
Yield Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.

Product FAQ


Q1: Can saliva be used as sample input?

Kit Components