Quick-cfRNA Serum & Plasma Kit


Quick-cfRNA Serum & Plasma Kit

Cat # Name Size Price Quantity
R1059 Quick-cfRNA Serum & Plasma Kit 50 preps $355.00
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R1059 Quick cfRNA Serum & Plasma-Kit

Highlights

  • Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
  • Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
  • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
Description

The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.


Elution Volume cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Type Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size RNA ≥ 17 nt.
Yield Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.

Q1: Can saliva be used as sample input?

Saliva must be cleared to remove debris/mucus (12,000xg for 15 min at room temperature). Up to 1 ml cleared salvia can be used per extraction.



Cat # Name Size Price
R1059-4-100 Quick-cfRNA Binding Buffer 100 ml $127.00
R1059-3-50 Quick-cfRNA Digestion Buffer 50 ml $127.00
R1003-3-12 RNA Wash Buffer 12 ml $30.00
R1070-1-10 RNA Recovery Buffer 10 ml $18.00
R1060-2-50 RNA Prep Buffer 50 ml $61.00
C1001-50 Collection Tubes 50 Pack $15.00
C1004-50 Zymo-Spin IC Columns 50 Pack $53.00
C1039-25 25 ml Reservoir for Spin-Away Filters and Zymo-Spin IIICG 25 Pack $41.00
C1006-50-F Spin-Away Filters 50 Pack $55.00
W1001-4 DNase/RNase-Free Water 4 ml $12.00