Quick-cfRNA Serum & Plasma Kit
- Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
- Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
|Elution Volume||cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.|
|Processing Time||Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.|
|Purity||High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.|
|Required Equipment||Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.|
|Sample Type||Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.|
|Size||RNA ≥ 17 nt.|
|Yield||Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.|