Quick-DNA 96 Kit
D3012 / D3011 / D3010
- Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
- Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
- Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
|Elution Volume||≥ 25 µl|
|Equipment||Microcentrifuge, vortex, centrifuge w/ microplate carriers|
|Purity||High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8|
|Sample Source||Whole blood, plasma, serum cultured cells, buccal cells, tissue already digested with Proteinase K or mechanically homogenized from human, mouse, rat, etc. are effectively processed using this kit.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered|
|Workflow||Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.|
|Yield||Up to 5 µg/well total DNA is eluted into ≥30 µl DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.|
Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?
Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
Q3: Can the Quick-DNA kit be used with bacterial samples?
Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?
Q5: Can Quick-DNA process crude lysates?
Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Q7: Is it possible to add an RNase A treatment to the protocol?
Q8: What are the expected yields for each sample type?
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