Quick-DNA Fecal/Soil Microbe Miniprep Kit
- Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 50 µl|
|Equipment||Microcentrifuge, vortex, cell disruptor/pulverizer|
|Processing Volume||≤150 mg feces, ≤ 250 mg soil, ≤100 mg fungal/bacterial cells (wet weight)|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Feces or soil|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.|
|Yield||≤ 25 µg total DNA|
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q2: Are there any tips in optimzing bead beating conditions?
Q3: What is the purpose of Zymo-Spin III-HRC step?
Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q5: When can an RNase A treatment be implemented in the protocol?
Fecal DNA from swabs was extracted with the ZR Fecal DNA MiniPrep for library preparation and metagenomic sequencing. Results showed a substantial difference in the gut microbiome in which the metagenome of weaned animals were enriched in processes involving glycan deconstruction and consumption.Frese S, et. al. (2015). Diet shapes the gut microbiome of pigs during nursing and weaning. Microbiome. 3:28.
The ZR Fecal DNA MiniPrep was used to extract DNA from pig fecal samples to determine the effect of proanthocyanidins (PACS) ingestion on phenolic metabolites in the gut microbiome. Results showed a significant increase in the population of specific bacterial strains, attributing the relationship of PACs and colon health to the microbiome.Choy YY, et. al. (2014). Phenolic metabolites and substantial microbiome changes in pig feces by ingesting grape seed proanthocyandins . Food & Function. 5(9):2298-2308.
High-quality DNA was purified from Blastocystis positive fecal samples using the ZR Fecal DNA MiniPrep for the purpose of comparing detective sensitivity between PCR methods and other commercial products. DNA was determined to be inhibitor free and highly useable for PCR analysis with STS primers and a partial SSU rRNA gene, outperforming all other kits tested.Yoshikawa et. al (2011) Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples. Parasitol. Res.