Quick-DNA Fecal/Soil Microbe Midiprep Kit

D6110


Quick-DNA Fecal/Soil Microbe Midiprep Kit

D6110

Cat # Name Size Price Quantity
D6110 Quick-DNA Fecal/Soil Microbe Midiprep 25 Preps $475.00
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Documents


Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.

Highlights


  • Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.

Description


The Quick-DNA Fecal/Soil Microbe Midiprep Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 150 µl
Equipment Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter
Processing Volume ≤375 mg feces, ≤ 5 g soil, ≤ 500 mg fungal/bacterial cells (wet weight)
Purity A260/A280 nm ≥1.8.
Sample Source Feces or soil
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Type Total DNA
Yield ≤ 125 µg total DNA

Product FAQ


Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q2: What is the purpose of Zymo-Spin III-HRC step?

Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q4: When can an RNase A treatment be implemented in the protocol?

Kit Components