Quick-DNA Fecal/Soil Microbe Midiprep Kit
- Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
The Quick-DNA Fecal/Soil Microbe Midiprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 150 µl|
|Equipment||Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter|
|Processing Volume||≤375 mg feces, ≤ 5 g soil, ≤ 500 mg fungal/bacterial cells (wet weight)|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Feces or soil|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.|
|Yield||≤ 125 µg total DNA|
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q2: What is the purpose of Zymo-Spin III-HRC step?
Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q4: When can an RNase A treatment be implemented in the protocol?
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