Quick-DNA Fungal/Bacterial Midiprep Kit

D6105


Quick-DNA Fungal/Bacterial Midiprep Kit

D6105

Cat # Name Size Price Quantity
D6105 Quick-DNA Fungal/Bacterial Midiprep Kit 25 Preps $395.00
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Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Easily isolate DNA from tough-to-lyse fungi and bacteria.

Highlights


  • Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
  • Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
  • Simple: Fastest workflow (≤ 20 minutes).

Description


The Quick-DNA Fungal/Bacterial Midiprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 150 µl
Equipment Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter
Processing Time ≤ 20 minutes
Processing Volume ≤ 500mg fungi or bacteria (wet weight), 5x109 bacterial cells, 5x108 yeast cells, or 5x107 mammalian cells
Purity Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
Size Range Capable of recovering genomic DNA up to and above ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered.
Type Total DNA
Yield ≤ 125 µg total DNA

Product FAQ


Q1: Are there any tips in optimizing bead beating conditions?

Q2: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q4: When can an RNase A treatment be implemented in the protocol?

Kit Components