Description
The Quick-DNA Magbead Plus DNA Kit is the easiest method for high throughput total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo’s unique system allows for a simple Bind, Wash, & Elute procedure that is unmatched in providing ultra-pure and concentrated genomic DNA (> 50 kb) in as little as 60 minutes for 96 samples. Purified DNA is ready for quantification or applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Technical Specifications
| Elution Volume |
≤ 100 µl |
| Equipment |
Centrifuge fitted with a 96 well microplate carrier, heat block (55°C), 96-well magnetic rack, automated liquid handler (recommended), 2 mL 96 well plates and reagent carriers (user supplied). |
| Purity |
A260/A280 >1.8, A260/A230 >1.8 |
| Size Range |
Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| Workflow |
Utilizes a Proteinase K Digestion and magnetic binding bead technology for effective recovery of DNA from blood, cells, and tissues. |
| Yield |
Up to 10 µg per 50 µl of MagBinding Beads used |
Product FAQ
Q1: Can I use more sample input, than what’s recommended in the protocol, by scaling up the protocol?
Custom solutions can be provided, please contact automation@zymoresearch.com.
Q2: Do you have scripts available for your automated kits and/or do you provide scripting support?
Yes, we currently have scripts for Hamilton and KingFisher, as well as support for Tecan on some systems. The kit instruction manual includes an “automation guide” for users to script the protocol themselves and our automation support staff also provides automation troubleshooting and advice if needed.
For detailed help regarding all automation questions, please contact automation@zymoresearch.com.
Kit Components
| Cat # |
Name |
Size |
Price |
|
|
D3004-2-200
|
g-DNA Wash Buffer
|
200 ml
|
$54.00 |
|
|
D3004-4-16
|
DNA Elution Buffer
|
16 ml
|
$18.00 |
|
|
D3004-4-50
|
DNA Elution Buffer
|
50 ml
|
$32.00 |
|
|
D3004-5-250
|
DNA Pre-Wash Buffer
|
250 ml
|
$71.00 |
|
|
D3004-5-50
|
DNA Pre-Wash Buffer
|
50 ml
|
$26.00 |
|
|
D4068-2-22
|
Solid Tissue Buffer (Blue)
|
22 ml
|
$38.00 |
|
|
D4100-2-12
|
MagBinding Beads
|
12 ml
|
$125.00 |
|
|
D4100-2-6
|
MagBinding Beads
|
6 ml
|
$69.00 |
|
|
D4077-1-150
|
Quick-DNA MagBinding Buffer
|
150 ml
|
$88.00 |
|
|
D4081-1-200
|
BioFluid & Cell Buffer II
|
200 ml
|
$250.00 |
|
|
D4081-1-50
|
BioFluid & Cell Buffer II
|
50 ml
|
$66.00 |
|
|
D4081-2-22
|
Solid Tissue Digestion Buffer II
|
22 ml
|
$38.00 |
|
|
D4081-2-6
|
Solid Tissue Digestion Buffer II
|
6 ml
|
$11.00 |
|
