Quick-DNA Microprep Kit

D3021 / D3020


Quick-DNA Microprep Kit

D3021 / D3020

Cat # Name Size Price Quantity
D3021 Quick-DNA Microprep Kit 200 Preps $320.00
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D3020 Quick-DNA Microprep Kit 50 Preps $98.00
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Documents


Rapid purification of DNA from cells and whole blood.

Highlights


  • Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
  • Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
  • Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Description


The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

Technical Specifications


Elution Volume ≥10 µl
Equipment Microcentrifuge, Vortex
Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8
Sample Source Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered
Workflow Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.
Yield Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood yields 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.

Product FAQ


Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?

Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?

Q3: Can the Quick-DNA kit be used with bacterial samples?

Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?

Q5: Can Quick-DNA process crude lysates?

Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?

Q7: Is it possible to add an RNase A treatment to the protocol?

Q8: What are the expected yields for each sample type?

Reviews


“It was easy to work with, protocol easy to follow”

- Tinatin T.

“This kit did a good job of prepping clean genomic DNA.”

- Tara N. (United States Agricultural Research Service)

Kit Components