D3024 / D3025
- Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
- Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
- Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
|Elution Volume||≥50 µl|
|Purity||High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8|
|Sample Source||Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered|
|Workflow||Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.|
|Yield||Up to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.|
Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?
Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
Q3: Can the Quick-DNA kit be used with bacterial samples?
Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?
Q5: Can Quick-DNA process crude lysates?
Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Q7: Is it possible to add an RNase A treatment to the protocol?
Q8: What are the expected yields for each sample type?
The Quick-gDNA MiniPrep enabled the recovery of high quality DNA from blood pathogens for whole genome analysis of bacterium that cannot be cultured in vitro. The sequenced genome provided clues to the mechanism of pathogenicity.do Nascimento NC, Guimaraes AM, Santos AP, SanMiguel PJ, Messick JB. Complete genome sequence of Mycoplasma haemocanis strain Illinois. J Bacteriol. 2012;194(6):1605-6.
The Quick-gDNA MiniPrep kit was used to extract inhibitor-free DNA from the blood of pigs infected with Mycoplasma suis. The purified DNA was used in the development of a qPCR assay that is highly sensitive and specific for M. suis detection and quantification.Guimarares AM, et al. (2011) A quantitative TaqMan PCR assay for the detection of Mycoplasma suis. J Appl Microbiol. 111(2):417-25.
“It was easy to work with, protocol easy to follow”
- Tinatin T.
“This kit did a good job of prepping clean genomic DNA.”
- Tara N. (United States Agricultural Research Service)
"This product was amazing! I've used the same type of kit (quick DNA extract) from Sigma and this was far more superior. I used the same amount of postnatal tissue as I would have for the Sigma kit, however the yield I obtained from Zymo was quite astounding considering the time of tissue digestion. Secondly, the gDNA was much 'cleaner' upon measurement with our NanoDrop! "