Quick-DNA/RNA Pathogen MagBead

R2145 / R2146


Quick-DNA/RNA Pathogen MagBead

R2145 / R2146

Cat # Name Size Price Quantity
R2145 Quick-DNA/RNA Pathogen MagBead 1 x 96 Preps $293.00
+ -
R2146 Quick-DNA/RNA Pathogen MagBead 4 x 96 Preps $1,069.00
+ -

Documents


Pathogen DNA/RNA isolation from vectors, tissue and biological samples.

Highlights


  • High-throughput, magnetic-bead based purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.).
  • High-quality DNA/RNA is ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
  • DNA/RNA Shield included for sample collection, inactivation and storage.

Description


Quick-DNA/RNA Pathogen MagBead kit is designed for high-throughput purification of pathogen (virus, bacteria, protozoa) DNA and RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.) collected, transported and stored in DNA/RNA Shield™. DNA/RNA Shield is used for nucleic acid preservation and inactivation of pathogens.

The kit features a storage/lysis buffer system and can be combined with high density ZR BashingBead™ Lysis Tubes (*recommended) to facilitate complete homogenization of hard-to-lyse samples for efficient nucleic acid isolation. Small (>50 nt) and large (> 200 kb) DNA and RNA are bound to magnetic beads, washed and then eluted.

The isolated high-quality nucleic acids are suitable for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/qPCR detection.

Technical Specifications


Binding Capacity 10 µg DNA/RNA per 20 µl ZymoBIOMICS MagBinding Beads.
Elution Volume ≤ 50 µl ZymoBIOMICS DNase/RNase-Free Water
Equipment Needed Magnetic stand or separator, liquid handler or robotic sample processer (user provided).
Materials (avaliable separately):
ZR BashingBead Lysis Tubes (S6014; 0.1/2.0 mm)
ZR-96 MagStand (P1005)
Collection Plate (C2002; capacity 1.2 ml/well)
96-Well Block (P1001; capacity 2 ml/well)
Elution Plate (C2003; capacity 0.35 ml/well)
Cover Foil (C2007; 2, 6, 12 or 24 pack)
Purity High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
Sample Type ≤ 10 mg vectors (mosquitoes, fleas, ticks, other tough-to-lyse insects) and tissue types (animal tissue, plants, other hosts) or ≤ 200 µl biological liquids.
Size 50 nt to ~200 kb
Yield 10 µg DNA/RNA binding capacity (per 20 µl MagBinding Beads), ≥50 µl elution volume

Citations


Kit Components