Quick-DNA Urine Kit
- Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.
- Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to 1 month at ambient temperature.
- Zymo-Spin column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA sequencing, arrays, and DNA methylation analysis.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation sequencing.|
|Equipment||Centrifuge, microcentrifuge, and heat block/bath.|
|Processing Volume||≤ 40 ml|
|Purity||Typical A260/A280 ≥ 1.8|
|Sample Storage||Eluted DNA should be stored at ≤ -20°C.|
|Size Range||100 bp - 23 kb|
|Type||Total DNA including cfDNA|
|Yield||Up to 5 µg total DNA can be eluted into ≥ 10 µl. 6 - 1000 ng/ml for healthy females. 2 - 20 ng/ml for healthy males.|
Q1: If I plan to process my urine samples immediately, is the Urine Conditioning Buffer necessary?
Q2: What is the function of the Clearing Beads?
Q3: Can I process urine samples stabilized in DNA/RNA Shield?
Q4: What should I do if I see an oil layer on top of the pellet, after centrifugation?
Q5: Can cell-free DNA be isolated from urine stored at – 80 °C?
Q6: Is the Quick-DNA Urine kit compatible with DNA Urine Preserve from Streck?
DNA was extracted from urinary epithelial cells, non-invasively available tissue, using the Quick-DNATM Urine Kit in study of the Old Order Amish population in the US suspected of having mitochondrial DNA mutations. Collected urine samples were assessed for heteroplasmy level for the m.3243A>G mutation using high-resolution melt (HRM) profiling and confirmed through DNA Sanger sequencing. This finding represents the first report of mitochondrial respiratory chain disorders in the Amish community and suggests that it may be under-diagnosed.Lina GG, Amy G, Vockley CW, Dobrowolski SF, Biery A, Irani A, Ibarra J, Morton DH, Mohsen AW, Vockley J. (2016). Mitrocontrial respitory chain disorders in the Old Order Amish population
Researches used the Quick-DNA Urine Kit (previously known as Extract-all Urine DNA kit) to detect and investigate the role of tumor cell-free DNA copy number variations (CNVs) in urine as clinically relevant diagnostic and predictive biomarkers for metastatic hormone sensitive and metastatic castrate resistant stages of prostate cancer. Urine cfDNAs derived from Quick-DNA Urine Kit were used to perform whole genome sequencing to generate high quality sequencing library. Researchers were able to detect CNVs in all samples, furthermore they were able to detect specific urine cfDNAs genomic aberrations at loci of PTEN, TMPRSS2, AR, and NOTCH1. CNV changes after treatment (androgen deprivation therapy and doxetacel chemotherapy) allowed for the identification of treatment-associated CNV changes, most important of which have been reported to be aberrant in prostate cancer such as copy number changes in RNF43 and ZNRF3 loci and in the LGR4 and MYC proto oncogene loci. These findings could prove potential predictive and prognostic classifiers for advance prostate cancer patients.Author Cited
Good yields of DNA in a very simple efficient process.
- R. W. (Medical University of South Carolina)
The kit is very useful as it lets us to analyze big volumes without an ultracentrifugation step.
- S. B.M. (University of Barcelona)
The protocol was easy to use/follow and it didn't take a great deal of time to get results.
- S. H. (South College School of Pharmacy)
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