Quick-RNA FFPE Miniprep


Quick-RNA FFPE Miniprep

Cat # Name Size Price Quantity
R1008 Quick-RNA FFPE Miniprep 50 preps $295.00
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Documents


Isolate RNA and small/miRNA from FFPE tissue.

Highlights

  • Easy Processing: Included Deparaffinization Solution for simple paraffin removal. No xylene necessary.
  • Improved Recovery: Optimized Proteinase K digestion and heat ensures maximum recovery.
  • High Quality: Non-crosslinked DNA-free RNA are ready for all downstream applications including PCR, Next-Gen Sequencing, etc.
Description

The Quick-RNA FFPE Kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples. The unique chemistries of the product have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin column technology. The result is high-quality total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.


Equipment Microcentrifuge, vortex, heat block/bath
Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2
Size Range Total RNA ≥ 17 nt
Yield 50 µg RNA (binding capacity), ≥25 µl (elution volume)

Cat # Name Size Price
W1001-10 DNase/RNase-Free Water 10 ml $19.00
D3067-1-20 Deparaffinization Solution 20 ml $22.00
C1001-50 Collection Tubes 50 Pack $15.00
R1060-2-25 RNA Prep Buffer 25 ml $40.00
R1060-1-50
R1003-3-24 RNA Wash Buffer 24 ml $59.00
C1078-50 Zymo-Spin IICR Columns 50 Pack $55.00
D3050-1-5
E1010-1-4 DNA Digestion Buffer 4 mL $15.00