Quick-RNA Plant Kit
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from a wide variety of plant samples.
- Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
The Quick-RNA Plant Miniprep Kit provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.). For purification of total RNA including small RNAs (~50 µg), the Quick-RNA Plant Miniprep features a specially formulated RNA Lysis Buffer. The Zymo-Spin IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently adsorbs total RNA. The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin III-HRC Spin Filters. The RNA is suitable for use in various subsequent procedures including RT-PCR.
|Equipment||Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)|
|Purity||RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.|
|Sample Source||Leaves, stems, buds, flowers, fruit, seeds, etc.|
|Size Range||Total RNA ≥ 17 nt|
|Yield||50 µg RNA (binding capacity), ≥25 µl (elution volume)|